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Merck
CN

1.00092

FRASER Listeria Ammonium iron (III) Supplement

for Listeria spp., pkg of 10 vials, for the preparation of Half FRASER (Demi FRASER) Broth (Base) or FRASER Broth (Base)

别名:

FRASER Listeria Ammonium iron(III) Supplement, Listeria Selective Supplement

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关于此项目

经验公式（希尔记法）:
C6H8O7 · xFe · xH3N
化学文摘社编号:
1185-57-5
UNSPSC Code:
41106212
EC Index Number:
214-686-6
NACRES:
NA.74
MDL number:
MFCD03099410

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产品名称

FRASER Listeria Ammonium iron (III) Supplement, for Listeria spp., pkg of 10 vials, for the preparation of Half FRASER (Demi FRASER) Broth (Base) or FRASER Broth (Base)

SMILES string

[Fe]21OC(=O)C(CC(=O)O2)(CC(=O)O1)O.N

InChI

1S/C6H8O7.Fe.H3N/c7-3(8)1-6(13,5(11)12)2-4(9)10;;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);;1H3/q;+3;/p-3

InChI key

FRHBOQMZUOWXQL-UHFFFAOYSA-K

agency

ISO 11290
ISO

storage temp.

15-25°C

suitability

Listeria spp.

Quality Level

200

form

lyophilized powder

potency

>2000 mg/kg LD50, oral (Rat)

composition

Ammonium iron (III) citrate, 250 mg per vial

packaging

pkg of 10 vials

technique(s)

microbiological culture: suitable

pH

6-8 (20 °C, 100 g/L in H2O)

solubility

1200 g/L

application(s)

food and beverages
microbiology
pathogen testing

compatibility

for use with GranuCult® Half FRASER (Demi FRASER) Broth (Base) (1.00025), GranuCult® FRASER Broth (Base) (1.10398), EcoCult® Half FRASER broth (Base) (1.40197)

相关类别

Analysis Note

Growth promotion test according to ISO 11133
Mixed inoculum of Listeria, E.coli and E.faecalis
Additionaly monoculture of E.coli and E.faecalis
Pre-enrichment in Half Fraser Broth, afterwards enrichment in Fraser Broth
Confirmation on ALOA®-Medium resp. Tryptic soy agar
Inoculum on reference medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
10 - 100
- Listeria monocytogenes ATCC 13932 (WDCM 00021): 10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013): ≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012): ≥ 1000
- Enterococcus faecalis ATCC 19433 (WDCM 00009): ≥ 1000
- Enterococcus faecalis ATCC 29212 (WDCM 00087): ≥ 1000
Blacking
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
+
- Listeria monocytogenes ATCC 13932 (WDCM 00021): +
- Escherichia coli ATCC 25922 (WDCM 00013): -
- Escherichia coli ATCC 8739 (WDCM 00012): -
- Enterococcus faecalis ATCC 19433 (WDCM 00009): -
- Enterococcus faecalis ATCC 29212 (WDCM 00087): -
Growth on ALOA medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
>10 CFU
- Listeria monocytogenes ATCC 13932 (WDCM 00021): >10 CFU
Colonies
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
bluegreen with non transparent zone
- Listeria monocytogenes ATCC 13932 (WDCM 00021): bluegreen with non transparent zone
Growth on Tryptic soy agar
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): <100 CFU
- Enterococcus faecalis ATCC 19433 (WDCM 00009): <100 CFU
Incubation:
Half Fraser Broth: 24 hours at 30°C, aerobic
Fraser Broth: 48 hours at 37°C, aerobic
Confirmation on ALOA-Medium: 48 hours at 37°C, aerobic
Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic

General description

For preparation of FRASER broth, two different supplements are needed: FRASER Listeria Selective Supplement and FRASER Listeria Ammonium Iron (III) Supplement. The ammonium iron (III) citrate promotes the growth of the Listeria spp., and together with esculin (a component of the FRASER broth base), permits the β-D-glucosidase detection in Listeria spp.

Other Notes

Replaces: 1000920017

Legal Information

ALOA is a registered trademark of Biolife Italiana S.r.l.
ECOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

FRASER Listeria Ammonium iron (III) Supplement is an additive for the preparation of Half FRASER (Demi FRASER) Broth (Base) (GranuCult® 1.00025, EcoCult® 1.40197) or FRASER Broth (Base) (1.10398) for the enrichment of Listeria spp.

存储类别

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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商品

Detection of Listeria monocytogenes in Food and Environmental Samples

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

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