The Methenamine silver plating kit acc. to Gomori - for the detection of argent-affine structures in histological tissue, is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin, for example histological sections of e. g. the kidney, the intestine, or the liver. The periodic acid Schiff (PAS) reaction is often used for the visualization of basal membranes, fungal elements, or other argentaffin structures in the tissue. However, the Methenamine silver plating acc. to Gomori results in better and richer-in-contrast visualization of these target structures.
In the first step of the silver plating, the Periodic acid oxidizes the 1,2-glycols in the tissue to aldehydes. Due to the incubation with Methenamin-borat silver nitrate solution, these aldehydes are reduced and in turn are responsible for the reduction of the silver ions to metallic silver. This silver will appear black. The subsequent treatment of the tissue with gold chloride intensifies the staining of the argent-affinic structures and concurrently reduces the background staining, and the washing with sodium thiosulfate solution will remove the excess of silver-gold complexes. Like this, the target structures will exhibit a dark brown to black color and all other structures will be counter-stained with light green SF.
The package is sufficient for up to 50 applications. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.