Quality Segment
agency
APHA, ISO 11290
reg. compliance
according to FDA (BAM)
sterility
sterile; γ-irradiated
form
medium granules (dehydrated (DCM))
manufacturer/tradename
ReadyBag®
packaging
pkg of 60 pieces, pkg of
technique(s)
microbiological culture: suitable
pH
7.0-7.4 (25 °C, 55 g/L in H2O)
solubility
55 g/L
application(s)
food and beverages
microbiology
pathogen testing
storage temp.
15-25°C
suitability
Listeria spp. (spp.)
General description
Readybag® Half FRASER broth with supplements come in pre-weighed and gamma-irradiated to speed up and simplify food pathogen testing routines. This helps to eliminate all the media preparation steps from the workflow. The Readybag® single-use pouches remove the necessity of weighing, autoclaving, supplement handling, and extra lab space or equipment. It only requires the addition of sterile water before incubation.
The high nutrient content and the large buffer capacity of the Readybag® Half FRASER broth (with supplements) create optimum growth conditions for Listeria spp. Lithium chloride, nalidixic acid, and acriflavine hydrochloride inhibit the growth of accompanying bacteria. Esculin and ammonium iron (III) citrate forms a black complex of esculetin with iron (III) ions and facilitates the detection of β-D-glucosidase activity. But this reaction is not exclusive to Listeria spp. Hence, every primary and secondary enrichment in Fraser broth must be sub-cultured on selective plating media.
The high nutrient content and the large buffer capacity of the Readybag® Half FRASER broth (with supplements) create optimum growth conditions for Listeria spp. Lithium chloride, nalidixic acid, and acriflavine hydrochloride inhibit the growth of accompanying bacteria. Esculin and ammonium iron (III) citrate forms a black complex of esculetin with iron (III) ions and facilitates the detection of β-D-glucosidase activity. But this reaction is not exclusive to Listeria spp. Hence, every primary and secondary enrichment in Fraser broth must be sub-cultured on selective plating media.
Application
Readybag® Half FRASER broth (with supplements) is suitable for the primary selective enrichment of Listeria spp. from food and animal feed, environmental samples, and other materials.
Packaging
Each sachet contains 12.5 g of granulated Half Fraser broth to which 225 mL of sterile water is added
Legal Information
READYBAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable