Merck
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1.02894

Millipore

GranuCult® plus Deoxycholate lactose agar

for coliforms, suitable for microbiology

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别名:
Coliform selective agar

形式

medium granules (dehydrated (DCM))

质量水平

包装

pkg of 500 g

制造商/商品名称

GranuCult® plus

储存条件

dry media (Keep tightly closed)

技术

microbiological culture: suitable

颜色

red-brown

pH值(酸碱度)

6.9-7.3 (25 °C, 57 g/L in H2O)

溶解性

57 g/L

应用

food and beverages
microbiology

储存温度

15-25°C

适用性

coliforms

一般描述

GranuCult® plus Deoxycholate lactose agar is a selective agar for the enumeration and isolation of Coliform bacteria from milk, water, ice cream, and other materials. The concentrations of deoxycholate and citrate are so low that the coliform bacteria can grow normally, whereas the accompanying Gram-positive bacteria are inhibited. Degradation of lactose to acid is detected by the pH indicator neutral red, which changes its color to red, and by a zone of precipitation caused by bile acid.

应用

GranuCult® plus Deoxycholate lactose agar is suitable for the enumeration and isolation of Coliform bacteria from milk, water, ice cream, and other materials.

特点和优势

  • GranuCult® offers superior granulated culture media
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

分析说明

Appearance (clearness): clear to slightly opalescent
Appearance (colour): red-brown
pH-value (25 °C): 6.9 - 7.3
Solidification behaviour (2 hrs., 45 °C): liquid
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 40 %
Recovery on test medium (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): ≥ 40 %
Recovery on test medium (Enterobacter cloacae ATCC 13047 (WDCM 00083)): ≥ 40 %
Recovery on test medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≥ 40 %
Recovery on test medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≥ 40 %
Recovery on test medium (Shigella flexneri ATCC 12022 (WDCM 00126)): ≥ 40 %
Recovery on test medium (Proteus mirabilis ATCC 14273): ≥ 40 %
Recovery on test medium (Enterococcus faecalis ATCC 11700): ≤ 0.01 %
Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 0.01 %
Colony colour (Escherichia coli ATCC 25922 (WDCM 00013)): red
Colony colour (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): pink/red
Colony colour (Enterobacter cloacae ATCC 13047 (WDCM 00083)): pink/red
Colony colour (Salmonella typhimurium ATCC 14028 (WDCM 00031)): colourless
Colony colour (Salmonella enteritidis ATCC 13076 (WDCM 00030)): colourless
Colony colour (Shigella flexneri ATCC 12022 (WDCM 00126)): colourless
Colony colour (Proteus mirabilis ATCC 14273): colourless
Precipitate (Escherichia coli ATCC 25922 (WDCM 00013)): +
Precipitate (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): +/poor
Precipitate (Enterobacter cloacae ATCC 13047 (WDCM 00083)): +/poor
Incubation: 24 hrs.; 35 °C; aerobic.

其他说明

Appearance of Colonies & the corresponding Microorganisms
  • Red, surrounded by a zone of precipitate: Lactose-positive: Escherichia coli
  • Pale with a pink center and surrounded by a zone of precipitate: Weakly lactose-positive: Enterobacter, Klebsiella, and others
  • Colorless: Lactose-negative: Salmonella, Shigella, Proteus, Pseudomonas, and other

附注

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法律信息

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 2


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