agency
ISO 6579
form
powder
technique(s)
microbiological culture: suitable
pH
7.0±0.2 (25 °C)
application(s)
food and beverages
pharmaceutical
storage temp.
15-25°C
suitability
selective and differential for Salmonella Shigella
Quality Level
General description
GranuCult® prime Salmonella Shigella (SS) Agar is a nutrient rich medium which is designed to isolate and differentiate Salmonella spp. from food, animal feed, water, and other test samples.
The presence of brilliant green, ox bile, high concentrations of thiosulfate and citrate largely inhibits the accompanying flora and swarming of Proteus spp, while allowing the growth of Salmonella spp. The synergistic reaction between thiosulfate and iron salt present in the media enables the growth of H2S positive black color colonies.
Lactose present in the media differentiates between the lactose positive and lactose negative microorganisms. Lactose fermenting microbes form an acid which is indicated by forming red colonies due to the presence of a pH indicator, neutral red. Peptones provide nitrogen, vitamins, amino acids, and minerals for growth and agar acts as a solidifying agent.
The presence of brilliant green, ox bile, high concentrations of thiosulfate and citrate largely inhibits the accompanying flora and swarming of Proteus spp, while allowing the growth of Salmonella spp. The synergistic reaction between thiosulfate and iron salt present in the media enables the growth of H2S positive black color colonies.
Lactose present in the media differentiates between the lactose positive and lactose negative microorganisms. Lactose fermenting microbes form an acid which is indicated by forming red colonies due to the presence of a pH indicator, neutral red. Peptones provide nitrogen, vitamins, amino acids, and minerals for growth and agar acts as a solidifying agent.
Application
GranuCult® prime Salmonella Shigella (SS) Agar is used to isolate and differentiate Salmonella spp. and Shigella spp from food, animal feed, water, and other test samples.
Analysis Note
- Salmonella
- spp. produce translucent colonies with or without black center and yellowish-brown colored medium or may appear as almost black colonies due to H2S production.
- Shigella spp. produce translucent colonies without black center and yellowish-brown colored medium.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
存储类别
11 - Combustible Solids
wgk
WGK 3
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