| Appearance (clearness) | clear |
| Appearance (colour) | red |
| pH-value (25 °C) | 6.9 - 7.3 |
| Typical composition (g/litre): Peptone 20.0; Sodium chloride 5.0; Bile salts no. 3 1.5; Sorbitol 10.0; Neutral red 0.03; Crystal violet 0.001; Agar-agar 15.0. |
Growth promotion test in accordance with ISO 11133.
Base tested with supplement Art. 1.09202. |
| Inoculum on reference medium (Escherichia coli ATCC 35150 (O157)) | 10 - 100 |
| Inoculum on reference medium (Escherichia coli O157:H7 ATCC 43894) | 10 - 100 |
| Inoculum on reference medium (Escherichia coli O157:H7 ATCC 43895) | 10 - 100 |
| Inoculum (Escherichia coli ATCC 11775 (WDCM 00090)) | 10⁴ |
| Inoculum (Escherichia coli ATCC 25922 (WDCM 00013)) | 10⁴ |
| Inoculum (Staphylococcus aureus ATCC 6538 (WDCM 00032)) | 10⁴ |
| Inoculum (Staphylococcus aureus ATCC 25923 (WDCM 00034)) | 10⁴ |
| Colony count (Escherichia coli ATCC 35150 (O157)) | |
| Colony count (Escherichia coli O157:H7 ATCC 43894) | |
| Colony count (Escherichia coli O157:H7 ATCC 43895) | |
| Recovery on test medium (Escherichia coli ATCC 35150 (O157)) | ≥ 50% |
| Recovery on test medium (Escherichia coli O157:H7 ATCC 43894) | ≥ 50% |
| Recovery on test medium (Escherichia coli O157:H7 ATCC 43895) | ≥ 50% |
| Growth (Escherichia coli ATCC 11775 (WDCM 00090)) | partly inhibition |
| Growth (Escherichia coli ATCC 25922 (WDCM 00013)) | partly inhibition |
| Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032)) | total inhibition |
| Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)) | total inhibition |
| Colony colour (Escherichia coli ATCC 35150 (O157)) | colourless |
| Colony colour (Escherichia coli O157:H7 ATCC 43894) | colourless |
| Colony colour (Escherichia coli O157:H7 ATCC 43895) | colourless |
| Colony colour (Escherichia coli ATCC 11775 (WDCM 00090)) | pink |
| Colony colour (Escherichia coli ATCC 25922 (WDCM 00013)) | pink |
Incubation: 24 hrs.; 37 °C; aerobic.
Reference: Tryptic-soy-agar. Inhibited strains tested with three-loop-streak. |
