SMAC agar base

for direct isolation and differentiation of enterohemorrhagic (EHEC) E.coli O157:H7-strains

Appearance (clearness)clearAppearance (colour)redpH-value (25 °C)6.9 - 7.3Typical composition (g/litre): Peptone 20.0; Sodium chloride 5.0; Bile salts no. 3 1.5; Sorbitol 10.0; Neutral red 0.03; Crystal violet 0.001; Agar-agar 15.0.Growth promotion test in accordance with ISO 11133.
Base tested with supplement Art. 1.09202.Inoculum on reference medium (Escherichia coli ATCC 35150 (O157))10 - 100Inoculum on reference medium (Escherichia coli O157:H7 ATCC 43894)10 - 100Inoculum on reference medium (Escherichia coli O157:H7 ATCC 43895)10 - 100Inoculum (Escherichia coli ATCC 11775 (WDCM 00090))10⁴Inoculum (Escherichia coli ATCC 25922 (WDCM 00013))10⁴Inoculum (Staphylococcus aureus ATCC 6538 (WDCM 00032))10⁴Inoculum (Staphylococcus aureus ATCC 25923 (WDCM 00034))10⁴Colony count (Escherichia coli ATCC 35150 (O157))Colony count (Escherichia coli O157:H7 ATCC 43894)Colony count (Escherichia coli O157:H7 ATCC 43895)Recovery on test medium (Escherichia coli ATCC 35150 (O157))≥ 50%Recovery on test medium (Escherichia coli O157:H7 ATCC 43894)≥ 50%Recovery on test medium (Escherichia coli O157:H7 ATCC 43895)≥ 50%Growth (Escherichia coli ATCC 11775 (WDCM 00090))partly inhibitionGrowth (Escherichia coli ATCC 25922 (WDCM 00013))partly inhibitionGrowth (Staphylococcus aureus ATCC 6538 (WDCM 00032))total inhibitionGrowth (Staphylococcus aureus ATCC 25923 (WDCM 00034))total inhibitionColony colour (Escherichia coli ATCC 35150 (O157))colourlessColony colour (Escherichia coli O157:H7 ATCC 43894)colourlessColony colour (Escherichia coli O157:H7 ATCC 43895)colourlessColony colour (Escherichia coli ATCC 11775 (WDCM 00090))pinkColony colour (Escherichia coli ATCC 25922 (WDCM 00013))pinkIncubation: 24 hrs.; 37 °C; aerobic.
Reference: Tryptic-soy-agar. Inhibited strains tested with three-loop-streak.