1.12018
Albumin fraction V
(from bovine serum) for biochemistry
别名:
Albumin fraction V, Bovine serum albumin, Serum albumin
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关于此项目
Biological source:
bovine
Form:
solid
Technique(s):
immunoassay: suitable
western blot: suitable
western blot: suitable
Concentration:
≥97% protein (spectralphotometric; calc. on anhydrous substance)
≥98% (Assay of Albumin (Agarose gel electrophoresis))
≥98% (Assay of Albumin (Agarose gel electrophoresis))
产品名称
Albumin fraction V, (from bovine serum) for biochemistry
biological source
bovine
form
solid
concentration
≥97% protein (spectralphotometric; calc. on anhydrous substance)
≥98% (Assay of Albumin (Agarose gel electrophoresis))
technique(s)
immunoassay: suitable
western blot: suitable
pH
6.8-7.2 (20 °C, 1 g/L in H2O)
solubility
1 g/L
bulk density
150 kg/m3
storage temp.
2-8°C
Analysis Note
Appearance (colour): slightly yellow
Appearance (description): leafs
Assay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 - 7.2
K (Potassium): ≤ 0.02 %
Na (Sodium): ≤ 0.5 %
Mg (Magnesium): ≤ 0.01 %
Ca (Calcium): ≤ 0.05 %
Heavy metals (as lead): ≤ 0.001 %
Fats: ≤ 0.2 %
Decomposition products: not detectable
Water (according to Karl Fischer): ≤ 5.00 %
Ash (600 °C): ≤ 1 %
NADH Oxidase (NADH; pH 7.5; 25° C): not detectable
LDH (pyruvate; pH 7.5; 25° C): not detectable
Appearance (description): leafs
Assay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 - 7.2
K (Potassium): ≤ 0.02 %
Na (Sodium): ≤ 0.5 %
Mg (Magnesium): ≤ 0.01 %
Ca (Calcium): ≤ 0.05 %
Heavy metals (as lead): ≤ 0.001 %
Fats: ≤ 0.2 %
Decomposition products: not detectable
Water (according to Karl Fischer): ≤ 5.00 %
Ash (600 °C): ≤ 1 %
NADH Oxidase (NADH; pH 7.5; 25° C): not detectable
LDH (pyruvate; pH 7.5; 25° C): not detectable
Application
Albumin fraction V has been used:
- as a component of perfusion buffer for perfusion protocol for rodent organ perfusion
- as a blocking agent in western blot analysis
- as a nitrated protein standard to study the effects of antibodies against 3-nitrotyrosine and to characterize the binding and detection of nitrated proteins in one-sided and sandwich immunoassays
Biochem/physiol Actions
Bovine Serum Albumin (BSA) is the chief component of cell culture media and also works as a blocking agent in enzyme-linked immunosorbent assay (ELISA). It is beneficial for embryonic stem cells (hESC) differentiation and assists the transport of drugs, hormones, and fatty acids.
General description
Bovine serum albumin (BSA) belongs to the serum albumin family with three domains and two sub-domains each. This globular, α-helical, non-glycosylated protein has 17-disulfide bonds.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
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商品
BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.
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