form
medium granules (dehydrated (DCM))
manufacturer/tradename
GranuCult®
technique(s)
microbiological culture: suitable
pH
6.7-7.1 (25 °C, 36.7 g/L in H2O, after autoclaving)
solubility
36.7 g/L
application(s)
food and beverages
microbiology
pathogen testing
storage temp.
15-25°C
suitability
Escherichia coli
Quality Level
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Analysis Note
Appearance (clearness): clear
Appearance (color): yellowish to yellowish-braun
pH-value (25 °C): 6.7 - 7.1
Inoculum on reference medium (Escherichia coli ATCC 35150 (O157)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 11775 (WDCM 00090)): ≤ 100
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 10000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 10000
Inoculum on reference medium (Proteus hauseri ATCC 13315): ≥ 10000
Growth (Escherichia coli ATCC 35150 (O157)): >10 transparent colonies with a pale yellowish-brown appearance on SMAC agar
Growth (Escherichia coli ATCC 11775 (WDCM 00090)): >10 pink colonies on SMAC Agar
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition on TSA
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition on TSA
Mixed inoculum of E.coli, P.hauseri and E.faecalis
Additionaly monoculture of E.faecalis
Confirmation on SMAC agar resp. Tryptic soy agar
Incubation:
mEC broth: 18 - 24 hours at 41,5 ± 1 °C aerobic
Confirmation on SMAC agar resp. Tryptic Soy Agar: 18 - 24 hours at 35 ± 1 °C aerobic
Appearance (color): yellowish to yellowish-braun
pH-value (25 °C): 6.7 - 7.1
Inoculum on reference medium (Escherichia coli ATCC 35150 (O157)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 11775 (WDCM 00090)): ≤ 100
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 10000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 10000
Inoculum on reference medium (Proteus hauseri ATCC 13315): ≥ 10000
Growth (Escherichia coli ATCC 35150 (O157)): >10 transparent colonies with a pale yellowish-brown appearance on SMAC agar
Growth (Escherichia coli ATCC 11775 (WDCM 00090)): >10 pink colonies on SMAC Agar
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition on TSA
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition on TSA
Mixed inoculum of E.coli, P.hauseri and E.faecalis
Additionaly monoculture of E.faecalis
Confirmation on SMAC agar resp. Tryptic soy agar
Incubation:
mEC broth: 18 - 24 hours at 41,5 ± 1 °C aerobic
Confirmation on SMAC agar resp. Tryptic Soy Agar: 18 - 24 hours at 35 ± 1 °C aerobic
Other Notes
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 3
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