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安全信息

126593

Sigma-Aldrich

白蛋白、牛血清、组分V、RIA和ELISA级

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别名:
白蛋白、牛血清、组分V、RIA和ELISA级
CAS号:

等级

RIA grade

检测方案

≥98% (agarose gel electrophoresis)

形式

solid

manufacturer/tradename

Calbiochem®

储存条件

OK to freeze

杂质

≤3.0 ash
≤5.0% moisture

颜色

off-white

溶解性

0.15 M sodium chloride: 10 mg/mL

痕量阳离子

heavy metals: ≤10 ppm

运输

ambient

储存温度

2-8°C

一般描述

牛血清白蛋白(BSA)是一种α-螺旋状的非糖基化球状蛋白,含有17个二硫键。
牛血清白蛋白,组分V(RIA和ELISA级)是可用作稳定剂和载体蛋白的纯化血浆蛋白。在非变性条件下通过改良Cohn方法和辛酸热激处理制备。在150 mM NaCl中10%的pH值为6.8-7.4。

应用

牛血清白蛋白,组分V(RIA和ELISA级)已用于:
  • 在免疫组化(IHC)分析中用于胎盘组织切片的封闭
  • 作为Krebs缓冲液组分,用于体外葡萄糖刺激胰岛素分泌(GSIS)实验和药物/棕榈酸制备
  • 作为溶剂对照孵育小鼠全血,用于研究体内血小板聚集

生化/生理作用

牛血清白蛋白(BSA)是细胞培养基的重要成分,可运输脂肪酸、激素和药物。它是酶联免疫吸附试验(ELISA)中常用的封闭剂。

警告

毒性:标准处理(A)

重悬

在复溶后,分装并冷冻保存(-20°C)。储备溶液在-20°C下可稳定保存至多6个月。

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

监管及禁止进口产品

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Katherine Paschalidou et al.
The Biochemical journal, 382(Pt 3), 1031-1038 (2004-07-06)
The development of renin inhibitors for the treatment of hypertension requires highly sensitive substrates to evaluate potency and to characterize the mechanism of tight-binding inhibitors. A series of intramolecularly quenched fluorogenic renin substrates, based on the N-terminal tetradecapeptide sequence of
Ernest T Parker et al.
Bio-protocol, 11(20), e4195-e4195 (2021-11-12)
Diffusion is a fundamental process in biological systems that governs the molecular collisions driving biochemical reactions and membrane and transport. Measurement of the diffusion coefficient and application of the Stokes-Einstein equation produces the hydrodynamic radius, which is a commonly used
Qi Da et al.
Microcirculation (New York, N.Y. : 1994), 25(6), e12457-e12457 (2018-04-28)
Monitoring endogenous platelets during intravital microscopy often involves two approaches: fluorescently labeled antibodies or genetic models of platelet-specific fluorescent protein expression. Due to limited data available on platelet functional changes induced by these methods, we compared functional effects of these
Jessica Suagee-Bedore et al.
Animals : an open access journal from MDPI, 11(12) (2021-12-25)
Older horses and those prone to obesity may be at a higher risk for inflammation than younger and leaner counterparts. Previous research indicated a postprandial elevation in plasma concentrations of interleukin-1β (IL-1β), a pro-inflammatory cytokine, after consuming 1.2 g of
Lucas A J Ahrens et al.
PloS one, 12(4), e0175095-e0175095 (2017-04-06)
Controlled release drug delivery systems are well established as oral and implantable dosage forms. However, the controlled release paradigm can also be used to present complex soluble signals responsible for cellular organization during development. Endochondral ossification (EO), the developmental process

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