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Merck
CN

126615

Sigma-Aldrich

Bovine Serum Albumin

10% Aqueous Solution

别名:

Albumin, Bovine Serum, 10% Aqueous Solution

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MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.25
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产品名称

Albumin, Bovine Serum, 10% Aqueous Solution,

生物来源

bovine

质量水平

描述

Merck USA index - 14, 8468

表单

liquid

制造商/商品名称

Calbiochem®

储存条件

OK to freeze

浓度

1 g/10 mL (Aqueous Solution)

技术

ELISA: suitable
PCR: suitable
immunoblotting: suitable

杂质

≤0.02% Fatty acids
≤10 ppm Heavy metal
≤2.0% Ash

异质活性

Nuclease, none detected
Protease, none detected

运输

ambient

储存温度

2-8°C

一般描述

Purified chromatographically to reduce DNases and RNases to exceptionally low levels.
Useful for applications in which acetylated BSA is not desirable, such as in antibody dilution, DNA footprinting and gel shift assay, ELISA, enzyme assay, enzyme stabilization, immunoblotting, immunofluorescence, PCR, restriction enzyme reactions, RIA, probe-based diagnostics, radioactive quenching, and receptor binding studies. Purified chromatographically to reduce DNases and RNases to exceptionally low levels.

外形

10% aqueous solution, 0.22 µm-filtered, pH 6.8-7.2.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Toxicity: Standard Handling (A)

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Alan J Simmons et al.
STAR protocols, 3(3), 101570-101570 (2022-07-27)
In droplet-based single-cell RNA-sequencing (scRNA-seq) experiments, cells, along with some of their surrounding buffer and ambient material, are encapsulated into droplets for mRNA capture and barcoding. This protocol details the steps for human gut tissue dissociation using cold active protease
Timothy T Harden et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(3), 602-607 (2016-01-07)
Production of a messenger RNA proceeds through sequential stages of transcription initiation and transcript elongation and termination. During each of these stages, RNA polymerase (RNAP) function is regulated by RNAP-associated protein factors. In bacteria, RNAP-associated σ factors are strictly required
Larry J Friedman et al.
Cell, 148(4), 679-689 (2012-02-22)
Understanding the pathway and kinetic mechanisms of transcription initiation is essential for quantitative understanding of gene regulation, but initiation is a multistep process, the features of which can be obscured in bulk analysis. We used a multiwavelength single-molecule fluorescence colocalization
Larry J Friedman et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(24), 9740-9745 (2013-05-31)
Sequence-specific DNA binding proteins must quickly bind target sequences, despite the enormously larger amount of nontarget DNA present in cells. RNA polymerases (or associated general transcription factors) are hypothesized to reach promoter sequences by facilitated diffusion (FD). In FD, a
Larry J Friedman et al.
Biophysical journal, 91(3), 1023-1031 (2006-05-16)
Complexes of macromolecules that transiently self-assemble, perform a particular function, and then dissociate are a recurring theme in biology. Such systems often have a large number of possible assembly/disassembly intermediates and complex, highly branched reaction pathways. Measuring the single-step kinetic

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