SeQuant^® ZIC^®-HILIC^™ 200 Å (3.5 µm) HPLC columns

The SeQuant^® ZIC^®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC™-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization. The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions. The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes. Thanks to their favorable retention and robust performance, SeQuant^® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^® ZIC^®-HILIC for the analysis of melamine and related substances. SeQuant^® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.


Learn more about the Supelco^® HPLC Column portfolio and Method development:

Brochure: A Practical Guide to HPLC - Method Development Guide href="/deepweb/assets/sigmaaldrich/marketing/global/documents/600/187/hplc-columns-guide-br7614en-ms.pdf">HPLC and UHPLC Column Selection Guide" target="_blank">Brochure: HPLC and UHPLC Column Selection Guide

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

Supelco is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany