16-193
抗磷酸化组蛋白H2A.X（Ser139）抗体，克隆JBW301，生物素结合物
clone JBW301, Upstate®, from mouse
别名:
H2AXS139P, Histone H2A.X (phospho S139), H2A histone family, member X, H2AX histone
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
biotin conjugate
Clone:
JBW301, monoclonal
Application:
ICC, WB
Citations:
43
biological source
mouse
conjugate
biotin conjugate
antibody form
purified antibody
antibody product type
primary antibodies
clone
JBW301, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer139)
Quality Level
Gene Information
human ... H2AX(3014)
General description
15 kDa
组蛋白是一种碱性核蛋白，负责真核生物中染色体纤维的核小体结构。核小体由包裹在由四个核心组蛋白（H2A，H2B，H3和H4）中的每一个对组成的组蛋白八聚体周围的大约146 bp的DNA组成。染色质纤维通过接头组蛋白H1的相互作用进一步压实，DNA在核小体之间形成更高阶的染色质结构。
Immunogen
对应于人组蛋白H2A.X氨基酸134-142的KLH偶联，合成肽（CKATQA[pS]QEY）免疫序列在酵母和小鼠中具有8个相同氨基酸。
表位：Ser139
Application
抗磷酸组蛋白H2A.X（Ser139）抗体，克隆JBW301，生物素偶联物是一种小鼠单克隆抗体，可用于检测磷酸组蛋白H2A.X（Ser139）（也被称为H2AXS139P，H2AX组蛋白） & 已在WB & PIA中进行了验证。
研究子类别
组蛋白
组蛋白
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
基于同源序列的保守型，预测其会发生广泛的物种交叉反应。
识别Ser139磷酸化的组蛋白H2A.X，Mr 15kDa。
Physical form
纯化生物素小鼠单克隆IgG1，溶于含有70％储存缓冲液（0.02 M磷酸盐缓冲液，pH7.6，0.25 M NaCl，0.1%叠氮化钠）和30％甘油的缓冲液中
蛋白G纯化
Preparation Note
自收到之日起在-20ºC可稳定保存1年。
使用建议：收到后，在取下瓶盖之前，将小瓶离心并轻轻混合溶液。分装到微量离心管中，并储存于-20°C。避免反复冻融，其可能会破坏IgG并影响产品性能。注意：冷藏室温度变化至低于-20°C时可能导致含甘油的溶液在储存过程中冻结。
使用建议：收到后，在取下瓶盖之前，将小瓶离心并轻轻混合溶液。分装到微量离心管中，并储存于-20°C。避免反复冻融，其可能会破坏IgG并影响产品性能。注意：冷藏室温度变化至低于-20°C时可能导致含甘油的溶液在储存过程中冻结。
Analysis Note
在用0.5 mM星形孢菌素（19-123）处理的Jurkat细胞裂解物中酸提取的组蛋白中进行常规评估。
蛋白质印迹分析：
0.5-1 μg/mL的该抗体在5 μM星形孢菌素（目录号19-123）处理过的Jurkat细胞裂解物中酸提取的组蛋白中检测到磷酸化的组蛋白H2A.X（Ser139）。
蛋白质印迹分析：
0.5-1 μg/mL的该抗体在5 μM星形孢菌素（目录号19-123）处理过的Jurkat细胞裂解物中酸提取的组蛋白中检测到磷酸化的组蛋白H2A.X（Ser139）。
对照
星形孢菌素处理的Jurkat细胞裂解物。
星形孢菌素处理的Jurkat细胞裂解物。
Other Notes
浓度：请参考批次特异性浓缩物的检验报告。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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