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Merck
CN

17-295

染色质免疫沉淀(ChIP)检测试剂盒

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

别名:

Agarose ChIP Kit, ChIP, Chromatin enrichment, chromatin immunoprecipitation

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UNSPSC Code:
12161503
NACRES:
NA.41
eCl@ss:
32161000
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产品名称

染色质免疫沉淀(ChIP)检测试剂盒, Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

Quality Level

manufacturer/tradename

EZ-ChIP

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

相关类别

Analysis Note

通过使用抗乙酰基组蛋白 H4 多克隆抗体(06-866)免疫沉淀乙酰化的组蛋白 H4 进行了常规评估。 随后使用相同的抗体进行了免疫印迹分析。

General description

用于富集染色质的特定区域,该区域可以用染色质蛋白的特异性抗体进行免疫沉淀。适用于所有真核细胞类型。 研究人员必须凭经验确定检测目的基因或启动子的染色质免疫沉淀反应。建议使用定量 PCR 检测。

Other Notes

蛋白 A 琼脂糖/鲑鱼精子 DNA(货号16-157)

所有必需的缓冲液

Packaging

试剂盒容量:22 次测定

Physical form

包含进行 22 次独立的染色质免疫沉淀(ChIP)反应所需的所有试剂。 提供的缓冲液足以从多达五个 15 cm 平板的培养细胞上产生染色质,每个平板可提供多达 10 个染色质制剂(随细胞和测定类型的不同而不同)。

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Adjacent sequence controls the response polarity of nitric oxide-sensitive Sp factor binding sites
Zhang, J., et al
The Journal of Biological Chemistry, 278, 29192-29200 (2003)
Orexin signaling mediates the antidepressant-like effect of calorie restriction.
Lutter, M; Krishnan, V; Russo, SJ; Jung, S; McClung, CA; Nestler, EJ
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Xu, XF; Ma, XL; Shen, Z; Wu, XL; Cheng, F; Du, LZ
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蛋白质是细胞内部的主要功能分子,负责细胞功能和生存的多种生物学活动。值得关注的是,许多蛋白质也能与DNA发生相互作用。

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

The 3rd edition of An Introduction to Antibodies and Their Applications provides a concise overview of some of the key features for the use of antibodies and immunochemical techniques in biological research. This handy reference guide supplements the techniques described in literature, recorded in general laboratory procedures, and described on individual product data sheets. Antibody design, development, and production are our expertise. Stringent validation of our antibodies is only one component of a comprehensive process we undertake to provide the antibodies most cited by the research community (see section Antibody Quality on page 2 for an in-depth look at our expertise).

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