203325
BMS
Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT.
别名:
BMS, bis(2-Mercaptoethylsulfone)
SMILES string
[S](=O)(=O)(CCS)CCS
InChI
1S/C4H10O2S3/c5-9(6,3-1-7)4-2-8/h7-8H,1-4H2
InChI key
YWXCBLUCVBSYNJ-UHFFFAOYSA-N
assay
≥99% (titration)
form
crystalline solid
reaction suitability
reagent type: reductant
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white
pKa
(1) 7.9, (2) 9.0
shipped in
ambient
storage temp.
−20°C
Quality Level
General description
Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT. Shown to reduce accessible disulfide bonds in immunoglobulin and trypsinogen under non-denaturing conditions 5 to 7 times faster than DTT at pH 7. The relatively less accessible disulfide bond in α-chymotrypsinogen A was reduced 2.3 times faster at pH 7 with BMS than with DTT. Has a pKa of 7.9 and 9.0.
Packaging
Packaged under inert gas
Preparation Note
May require warming (40-45°C) to achieve complete solubilization in H₂O.
Other Notes
Singh, R., et al. 1995. Methods Enzymol.251, 167.
Singh, R., and Whitesides, G.M. 1994. Bioorg. Chem.22, 109.
Singh, R., and Whitesides, G.M. 1994. Protein Sci. 3, Suppl. 1, 430T.
Lamoureux, G.V., and Whitesides, G.M. 1993. J. Org. Chem.58, 633.
Singh, R., and Whitesides, G.M. 1994. Bioorg. Chem.22, 109.
Singh, R., and Whitesides, G.M. 1994. Protein Sci. 3, Suppl. 1, 430T.
Lamoureux, G.V., and Whitesides, G.M. 1993. J. Org. Chem.58, 633.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
监管及禁止进口产品
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