产品名称
抗-CD44大鼠mAb (A020), liquid, clone A020, Calbiochem®
biological source
rat
antibody form
purified antibody
antibody product type
primary antibodies
clone
A020, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
canine, bovine, human, rabbit, porcine, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(ELISA (1:2000)
Flow Cytometry (1:1000)
Immunoblotting (1:1000)
Immunofluorescence (1:200)
Immunohistochemistry (1:250)
Immunoprecipitation (1:200)
Neutralization Studies
Radioimmunoassay (1:5000))
isotype
IgG2b
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
bovine ... Cd44(281057)
dog ... Cd44(403939)
human ... CD44(960)
mouse ... Cd44(12505)
pig ... Cd44(100525150)
General description
抗CD44,大鼠单克隆,克隆A020,可识别人的~85-95 kDa CD44蛋白(gp90)和小鼠的GP85。经验证可用于ELISA、IF、IP、RIA、WB和IHC。
通过硫酸铵沉淀和DEAE色谱法纯化的大鼠单克隆抗体。识别~85-95 kDa CD44蛋白。
可识别人的约85-95 kDa CD44蛋白(gp90)和小鼠的GP85或Pgp-1。
Immunogen
人
纯化人淋巴细胞CD44
Other Notes
Iida, N., and Bourguignon, L.Y.W.1995.J. Cell Physiol.162, 121.
Bourguignon, L.Y.W., et al. 1993.J. Immunol.151, 6634.
Bourguignon, L.Y.W., et al. 1993.J. Immunol.151, 6634.
该抗体可特异性识别CD44类糖蛋白的共同决定簇并与之反应。据报道,该抗体可阻断透明质酸和CD44之间的相互作用(请参阅应用参考)。合适的工作稀释度由与测定条件相关的变量决定。
Packaging
请参考特定浓度批号的标签。
Physical form
在150 mM NaCl,10 mM磷酸钠(pH 7.5)中。
Preparation Note
初次融化后,等分并冷冻(-20°C)。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Application
ELISA(1:2000)
流式细胞仪(1:1000)
免疫印迹(1:1000)
免疫荧光(1:200)
免疫组织化学(1:250)
免疫沉淀(1:200)
中和研究(请参阅注释和应用参考)
放射免疫分析(1:5000)
流式细胞仪(1:1000)
免疫印迹(1:1000)
免疫荧光(1:200)
免疫组织化学(1:250)
免疫沉淀(1:200)
中和研究(请参阅注释和应用参考)
放射免疫分析(1:5000)
Disclaimer
毒性:标准处理(A)
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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