biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
IB4, monoclonal
assay
≥95% (SDS-PAGE)
form
liquid
contains
0.5 mg/mL gentamicin sulfate as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Flow Cytometry (5-10 µg/mL)
Frozen Sections
Immunoprecipitation
Cell Inhibition Assays )
isotype
IgG2a
shipped in
wet ice
Quality Level
target post-translational modification
unmodified
General description
抗-CD18，小鼠单克隆，克隆IB4，可识别~95 kDa β2整联蛋白亚基。它已针对冷冻切片上的 FC、IP、IHC和细胞抑制试验进行了验证。
蛋白A纯化的小鼠单克隆抗体。识别~95 kDa β2整联蛋白亚基（CD18）。
识别~95 kDa β2整联蛋白亚基。阻断ICAM-1和ICAM-3与LFA-1的结合。
Immunogen
人
人外周血血单核细胞
Application
流式细胞术（5-10 µg/ml）
冷冻切片（参见注释）
免疫沉淀（参见注释）
细胞抑制试验（参见注释）
冷冻切片（参见注释）
免疫沉淀（参见注释）
细胞抑制试验（参见注释）
Packaging
请参考特定浓度批号的标签。
Physical form
在150 mM NaCl，100 mM KCl，50 mM磷酸钠中，pH 7.5。
Preparation Note
初次使用后，分装冻存（-20°C）以进行长期储存。避免冻/融循环。
Analysis Note
阳性对照
HPB-MLT细胞
HPB-MLT细胞
阴性对照
Nalm-6细胞
Nalm-6细胞
Other Notes
Berman, M.E., et al. 1996.J. Immunol.156, 1515.
Kaneko, M., et al. 1995.J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995.Haematologica80, 161.
Kaneko, M., et al. 1995.J. Immunol.155, 2631.
Landis, R.C., et al. 1994.J. Cell Biol.126, 529.
Wright, S.D., et al. 1983.Proc.Natl.Acad.Sci. USA80, 5699.
Kaneko, M., et al. 1995.J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995.Haematologica80, 161.
Kaneko, M., et al. 1995.J. Immunol.155, 2631.
Landis, R.C., et al. 1994.J. Cell Biol.126, 529.
Wright, S.D., et al. 1983.Proc.Natl.Acad.Sci. USA80, 5699.
阻断ICAM-1和ICAM-3与LFA-1的结合。使用5 µg/ml的抗-CD18抗体和FITC偶联的山羊抗小鼠IgG对5x105培养的人HPB-MLT细胞进行FACS。据报道，该抗体也可用于免疫沉淀和冰冻切片。合适的工作稀释度由与测定条件相关的变量决定。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：标准处理（A）
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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