产品名称
抗CD28小鼠单克隆抗体（ANC28.1 / 5D10）, liquid, >95% (immunoglobulin, SDS-PAGE), clone ANC28.1/5D10
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
ANC28.1/5D10, monoclonal
assay
>95% (immunoglobulin, SDS-PAGE)
form
liquid
contains
500 μg/mL gentamycin sulfate as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Flow Cytometry (5 µg/mL)
Frozen Sections
Immunoprecipitation
T cell Co-stimulation )
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CD28(940)
Application
流式细胞术（5 µg/ml）
冷冻切片（参见评论）
免疫沉淀法（参见评论）
T 细胞共刺激（参见评论）
冷冻切片（参见评论）
免疫沉淀法（参见评论）
T 细胞共刺激（参见评论）
Disclaimer
毒性：标准处理（A）
General description
在人 T 细胞和转染了人 CD28 的 BHK 细胞上识别 CD28。
小鼠抗CD28单克隆抗体（克隆ANC28.1 / 5D10）识别人T细胞上的CD28 & 人CD28转染的BHK细胞。 已针对冰冻切片的FC，IP，IHC和T细胞共刺激进行了验证。
蛋白 A 纯化的小鼠单克隆抗体。识别 CD28 蛋白。
Immunogen
人
人 CD28 Ig 融合蛋白和 HPB-MLT 人肿瘤细胞
Other Notes
Kariv, I., et al. 1996.J. Immunol.157, 29.
61st Forum in Immunology.1995.Res. Immunol.146, 127.
Jenkins, M.K., and Johnson, J.G.1993.Curr.Opin.Immunol.5, 361.
61st Forum in Immunology.1995.Res. Immunol.146, 127.
Jenkins, M.K., and Johnson, J.G.1993.Curr.Opin.Immunol.5, 361.
单体和交联抗体会刺激 CD28+ 细胞表达 IL-2。使用 5 µg/ml 的抗 CD28 抗体和 FITC 偶联的山羊抗小鼠 IgG 对 5x105 培养的 HPB-MLT 细胞进行 FACS。也有报道表明该抗体可用于冷冻切片和免疫沉淀。合适的工作稀释度由与测定条件相关的变量决定。
Packaging
请参考特定浓度批号的标签。
Physical form
在 150 mM NaCl，100 mM KCl，50 mM 磷酸钠中，pH 7.5。
Preparation Note
初次使用后，分装冻存（-20°C）以进行长期储存。避免冻/融循环。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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