跳转至内容
Merck
CN

2502

ReBlot Plus温和抗体剥离液,10x

别名:

Western blot stripping solution, blot stripping solution

登录 查看组织和合同定价。

选择尺寸


关于此项目

eCl@ss:
42029053
UNSPSC Code:
41116010
NACRES:
NA.56
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

form

liquid

packaging

pkg of 50 mL

manufacturer/tradename

Chemicon®
Re-Blot

technique(s)

western blot: suitable

detection method

chemiluminescent

shipped in

wet ice

Legal Information

Sepharose is a trademark of Cytiva
Re-Blot is a trademark of Merck KGaA, Darmstadt, Germany
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

MILLIPORE Re-Blot Plus Western Blot温和抗体剥离液可有效去除已用化学发光法或放射性碘或其他同位素显影的蛋白质印迹中的抗体。 不建议剥离比色底物(TMB、DAB、4-氯萘酚等),因为不可能有效去除在反应位点沉淀的底物。

在通过比较印迹分析确定剥离不会定量影响给定抗原之前,Re-Blot Plus Western Blot温和抗体剥离溶液应仅用于定性。

本品仅供研究使用;不用于诊断或体内使用。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

免疫印迹是研究蛋白质功能和定位的常用技术。通常,将蛋白样品在SDS-PAGE上进行电泳,然后转移到硝酸纤维素膜或尼龙膜上,然后在其中用特异性抗体进行探测。与可重复利用Southern和Northern印迹的核酸技术不同,免疫印迹很难重复使用。

免疫印迹的剥离和重新检测技术具有以下几个优点:

1)保护昂贵或限量供应的样品,

2)指定印迹分析可使用几种不同抗体,如亚型或同种型特异性抗体,

3)使用相同或不同的抗体重新分析异常结果并确认,

4)纠正使用错误的抗体孵育而产生的错误结果,

5)重复使用相同印迹可节省试剂和时间。

尽管基于抗原和抗体的免疫亲和基质(例如Sepharose偶联物)已被重复使用多次而不会损害抗原-抗体的反应性,但对极端pH值和离液剂的需求已排除了这些方法在蛋白质印迹中的应用。

MILLIPORE Re-Blot Plus Western Blot温和抗体剥离溶液包含特殊配制的溶液,可快速有效地从蛋白质印迹中去除抗体,而不会显著影响固定的蛋白质。

Re-Blot Plus Western Blot温和的抗体剥离液的优点包括:

  • 抗体剥离液中不含刺激性气味β-巯基乙醇。
  • 抗体剥离在室温下进行。无需加热印迹。
  • 可在室温下约15分钟内剥离抗体印迹。
  • 印迹可在25分钟内重复使用。

Other Notes

温和的抗体剥离液(10x)-(2个容器/每个25 mL)。

Preparation Note

温和的抗体剥离液应在到达后在2-8°C下保存。产品在接收后可稳定保存3至6个月。 如果抗体剥离液在储存时结晶,则可以在使用前在37°C下缓慢加热将其重新溶解。

注意:为防止试剂降解,在储存时盖紧瓶盖。 避免长时间暴露在空气中。

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

存储类别

6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Jannike M Andersen et al.
Behavioural brain research, 390, 112676-112676 (2020-05-15)
Activation of calcium/calmodulin-dependent protein kinase II (CaMKII), particularly its α isoform, is known to be important for neuronal processes central for learning and memory and has also been implicated in the maladaptive learning involved in drug addiction.Thr286 autophosphorylation of αCaMKII
Xiaoyu Wang et al.
Proteome science, 9, 53-53 (2011-09-16)
An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity
Cristian Mircean et al.
Bioinformatics (Oxford, England), 21(9), 1935-1942 (2005-01-14)
The protein lysate microarray is a developing proteomic technology for measuring protein expression levels in a large number of biological samples simultaneously. A challenge for accurate quantification is the relatively narrow dynamic range associated with the commonly used chromogenic signal
Nicholas W Baetz et al.
Molecular biology of the cell, 24(5), 643-658 (2013-01-04)
The Rab11-family interacting proteins (Rab11-FIPs) facilitate Rab11-dependent vesicle recycling. We hypothesized that Rab11-FIPs define discrete subdomains and carry out temporally distinct roles within the recycling system. We used live-cell deconvolution microscopy of HeLa cells expressing chimeric fluorescent Rab11-FIPs to examine
Alyssa M Fedorko et al.
Heliyon, 6(5), e04075-e04075 (2020-06-04)
Endometrial cancer is the most common gynecologic malignancy in the U.S. with metastatic disease remaining the major cause of patient death. Therapeutic strategies have remained essentially unchanged for decades. A significant barrier to progression in treatment modalities stems from a

相关内容

Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.

查看所有结果

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持