Mouse Anti-Human IgG₁, Fc Fragment Specific Alkaline Phosphatase Conjugate

Protein A purified mouse monoclonal antibody conjugated to alkaline phosphatase. Recognizes human IgG₁, Fc fragment.

This Mouse Anti-Human IgG₁, Fc Fragment Specific Alk-Phos Conjugate is validated for use in ELISA, Enzyme Immunoassay, Immunoelectrophoresis for the detection of Human IgG₁.

ELISA (see comments)

Enzyme Immunoassay (≥1:5000)

Immunoelectrophoresis (see comments)

Please refer to vial label for lot-specific concentration.

Toxicity: Standard Handling (A)

In 50 mM Tris, 1 mM MgCl₂, 50% glycerol, pH 8.0.

Monospecific for human IgG₁ Fc fragment as determined by immunoelectrophoresis and direct ELISA against normal human serum and normal human IgG. p-Nitrophenylphosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl₂ at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany