biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
secondary antibodies
clone
monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(ELISA
Enzyme Immunoassay (1:5000)
Immunoelectrophoresis )
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Protein A purified mouse monoclonal antibody conjugated to alkaline phosphatase. Recognizes human IgG1, Fc fragment.
This Mouse Anti-Human IgG₁, Fc Fragment Specific Alk-Phos Conjugate is validated for use in ELISA, Enzyme Immunoassay, Immunoelectrophoresis for the detection of Human IgG₁.
Application
ELISA (see comments)
Enzyme Immunoassay (≥1:5000)
Immunoelectrophoresis (see comments)
Enzyme Immunoassay (≥1:5000)
Immunoelectrophoresis (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM Tris, 1 mM MgCl₂, 50% glycerol, pH 8.0.
Other Notes
Monospecific for human IgG1 Fc fragment as determined by immunoelectrophoresis and direct ELISA against normal human serum and normal human IgG. p-Nitrophenylphosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable