401459
Mouse Anti-Human IgG1, Fc Fragment Specific Alkaline Phosphatase Conjugate
liquid, Calbiochem®
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关于此项目
NACRES:
NA.77
UNSPSC Code:
12352203
Clone:
monoclonal
Species reactivity:
human
Application:
—
Citations:
2
biological source
mouse
antibody form
purified antibody
antibody product type
secondary antibodies
clone
monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(ELISA
Enzyme Immunoassay (1:5000)
Immunoelectrophoresis )
isotype
IgG
shipped in
wet ice
Quality Level
target post-translational modification
unmodified
General description
Protein A purified mouse monoclonal antibody conjugated to alkaline phosphatase. Recognizes human IgG1, Fc fragment.
This Mouse Anti-Human IgG₁, Fc Fragment Specific Alk-Phos Conjugate is validated for use in ELISA, Enzyme Immunoassay, Immunoelectrophoresis for the detection of Human IgG₁.
Application
ELISA (see comments)
Enzyme Immunoassay (≥1:5000)
Immunoelectrophoresis (see comments)
Enzyme Immunoassay (≥1:5000)
Immunoelectrophoresis (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM Tris, 1 mM MgCl₂, 50% glycerol, pH 8.0.
Other Notes
Monospecific for human IgG1 Fc fragment as determined by immunoelectrophoresis and direct ELISA against normal human serum and normal human IgG. p-Nitrophenylphosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Hong Yu et al.
Frontiers in cellular and infection microbiology, 14, 1342621-1342621 (2024-02-19)
Murine research has revealed a significant role for antibody responses in protection against Chlamydia reinfection. To explore potential humoral immune markers of protection elicited by Chlamydia trachomatis (CT) antigens in humans in the context of presumed clinical correlates of protection
Shota Kobayashi et al.
Journal of neuroimmunology, 257(1-2), 102-106 (2013-01-15)
Autoantibody against nicotinic acetylcholine receptor (nAChR) α3 subunit has been implicated in the pathogenesis of paraneoplastic neurological syndrome. To examine the effect of anti-α3 subunit autoantibody on cell-surface nAChRs, we established human embryonic kidney 293 cells stably co-expressing α3 and
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