Purified mouse monoclonal antibody. Recognizes human IgG₁ Fc fragment.

This Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) is validated for use in ELISA for the detection of Human IgG₁, Fc Fragment Specific.

ELISA (see comments and application references)

Please refer to vial label for lot-specific concentration.

In PBS, pH 7.4.

Following initial use aliquot and freeze (-20°C).

Can be used as a capture antibody for ELISA at 10 µg/ml in PBS without carrier protein. Antibody should be titrated for optimal results in individual systems.



Cross-reactivity by ELISA against human myeloma proteins:

Human IgG₁: 100%

Human IgG₂: 0.1%

Human IgG₃: 0.1%

Human IgG₄: <0.02%

Human IgA: <0.1%

Human IgE: <0.1%

Human IgM: <0.1%



Cross-reactivity by ELISA against chimeric antibodies:

Human IgG₁: <0.01%

Human IgG₂: <0.01%

Human IgG₃: <0.01%

Human IgG₄: <0.01%

Human IgA: <0.01%

Human IgE: <0.001%

Human IgM: <0.01%

Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)