Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate

Purified mouse monoclonal antibody conjugated to biotin. Recognizes human IgG₃ hinge region.

This Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate is validated for use in ELISA for the detection of Human IgG₃, Hinge Specific.

Human

ELISA (1:500-1:2000, see comments)

Please refer to vial label for lot-specific concentration.

Toxicity: Standard Handling (A)

In PBS, 1% BSA, pH 7.4.

Following initial thaw, aliquot and freeze (-20°C).

Biotinylated with hydroxysuccinimide ester biotin. This biotin-labeled antibody is an extremely versatile reagent because of the high binding affinity for avidin and avidin-labeled compounds. Variables associated with assay conditions will dictate the proper working dilution.



Cross-reactivity by ELISA against human myeloma proteins:

Human IgG₁: ≤0.1%

Human IgG₂: ≤1%

Human IgG₃: 100%

Human IgG₄: ≤0.1%

Human IgA: ≤0.1%

Human IgE: ≤0.1%

Human IgM: ≤0.1%



Cross-reactivity by ELISA against chimeric antibodies:

Human IgG₁: <0.001%

Human IgG₂: <0.001%

Human IgG₃: 100%

Human IgG₄: <0.001%

Human IgA: <0.001%

Human IgE: <0.001%

Human IgM: <0.001%

Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Hamilton, R.G. 1987. Clin. Chem.33, 1707.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany