411483
Mouse Anti-Human IgG3, Hinge Specific (HP6047) Biotin Conjugate
liquid, clone HP6047, Calbiochem®
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关于此项目
NACRES:
NA.77
UNSPSC Code:
12352203
Clone:
HP6047, monoclonal
Species reactivity:
human
Application:
—
Citations:
2
biological source
mouse
antibody form
purified antibody
antibody product type
secondary antibodies
clone
HP6047, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA (1:500-1:2000))
isotype
IgG3
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
Purified mouse monoclonal antibody conjugated to biotin. Recognizes human IgG3 hinge region.
This Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate is validated for use in ELISA for the detection of Human IgG₃, Hinge Specific.
Immunogen
Human
Application
ELISA (1:500-1:2000, see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 1% BSA, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Biotinylated with hydroxysuccinimide ester biotin. This biotin-labeled antibody is an extremely versatile reagent because of the high binding affinity for avidin and avidin-labeled compounds. Variables associated with assay conditions will dictate the proper working dilution.
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: ≤0.1%
Human IgG2: ≤1%
Human IgG3: 100%
Human IgG4: ≤0.1%
Human IgA: ≤0.1%
Human IgE: ≤0.1%
Human IgM: ≤0.1%
Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: <0.001%
Human IgG2: <0.001%
Human IgG3: 100%
Human IgG4: <0.001%
Human IgA: <0.001%
Human IgE: <0.001%
Human IgM: <0.001%
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: ≤0.1%
Human IgG2: ≤1%
Human IgG3: 100%
Human IgG4: ≤0.1%
Human IgA: ≤0.1%
Human IgE: ≤0.1%
Human IgM: ≤0.1%
Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: <0.001%
Human IgG2: <0.001%
Human IgG3: 100%
Human IgG4: <0.001%
Human IgA: <0.001%
Human IgE: <0.001%
Human IgM: <0.001%
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Hamilton, R.G. 1987. Clin. Chem.33, 1707.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Hamilton, R.G. 1987. Clin. Chem.33, 1707.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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