Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025)

Purified mouse monoclonal antibody. Recognizes human IgG₄ Fc fragment.

This Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025) is validated for use in Enzyme Immunoassay for the detection of Human IgG₄, Fc Fragment Specific.

Enzyme Immunoassay (10 µg/ml, see application references)

Please refer to vial label for lot-specific concentration.

In PBS, pH 7.4.

Following initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.

Antibody should be titrated for optimal results in individual systems.



Cross-reactivity by ELISA against human myeloma proteins:

Human IgG₁: ≤0.1%

Human IgG₂: ≤0.04%

Human IgG₃: ≤0.02%

Human IgG₄: 100%

Human IgA: <0.1%

Human IgE: <0.1%

Human IgM: <0.1%



Cross-reactivity by ELISA against chimeric antibodies:

Human IgG₁: <0.01%

Human IgG₂: <0.01%

Human IgG₃: <0.01%

Human IgG₄: 100%

Human IgA: <0.01%

Human IgE: <0.01%

Human IgM: <0.01%

Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)