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Merck
CN

411492

Mouse Anti-Human IgG4, Fc Fragment Specific (HP6025)

liquid, clone HP6025, Calbiochem®

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关于此项目

NACRES:
NA.46
UNSPSC Code:
12352203
Clone:
HP6025, monoclonal
Species reactivity:
human
Application:
—
Citations:
4

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biological source

mouse

antibody form

purified antibody

antibody product type

secondary antibodies

clone

HP6025, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Enzyme Immunoassay (10 µg/mL, )

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

相关类别

General description

Purified mouse monoclonal antibody. Recognizes human IgG4 Fc fragment.
This Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025) is validated for use in Enzyme Immunoassay for the detection of Human IgG₄, Fc Fragment Specific.

Application

Enzyme Immunoassay (10 µg/ml, see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.

Other Notes

Antibody should be titrated for optimal results in individual systems.



Cross-reactivity by ELISA against human myeloma proteins:

Human IgG1: ≤0.1%

Human IgG2: ≤0.04%

Human IgG3: ≤0.02%

Human IgG4: 100%

Human IgA: <0.1%

Human IgE: <0.1%

Human IgM: <0.1%



Cross-reactivity by ELISA against chimeric antibodies:

Human IgG1: <0.01%

Human IgG2: <0.01%

Human IgG3: <0.01%

Human IgG4: 100%

Human IgA: <0.01%

Human IgE: <0.01%

Human IgM: <0.01%
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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D C Houpt et al.
Infection and immunity, 62(7), 2857-2864 (1994-07-01)
Serum obtained from normal human subjects contains antibodies reactive in an enzyme-linked immunosorbent assay with the glucuronoxylomannan (GXM) of Cryptococcus neoformans. The frequency of occurrence of class-specific antibodies among normal subjects was 28% for immunoglobulin G (IgG), 98% for IgM
S Sievers et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 47(8), 1057-1068 (2017-03-28)
Microarray-based component-resolved diagnostics (CRD) has become an accepted tool to detect allergen-specific IgE sensitization towards hundreds of allergens in parallel from one drop of serum. Nevertheless, specificity and sensitivity as well as a simultaneous detection of allergen-specific IgG4 , as
R G Keller et al.
Infection and immunity, 62(1), 215-220 (1994-01-01)
Previous studies found that normal human serum (NHS) contains an immunoglobulin G (IgG) antibody that mediates initiation of the classical complement pathway by nonencapsulated Cryptococcus neoformans. The present study used an enzyme-linked immunosorbent assay with whole nonencapsulated yeast cells as
H Lu et al.
Infection and immunity, 61(6), 2400-2407 (1993-06-01)
The aims of this study were to determine the immunodominant antigens of Actinobacillus actinomycetemcomitans serotype b (Aab) for the different immunoglobulin (Ig) classes and subclasses and to determine the relative levels of these different Igs in serum. Seropositive early-onset periodontitis

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