biological source
rabbit
Quality Level
antibody form
diluted serum
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA (1:500-1:1000)
Frozen Sections (1:50-1:500)
Immunoblotting (1:1000-1:10,000)
Paraffin Sections (1:50-1:500))
isotype
IgG
shipped in
wet ice
storage temp.
−70°C
target post-translational modification
unmodified
General description
兔多克隆抗体以稀释的血清形式提供。识别天然硫辛酸和与蛋白质共价连接的硫辛酸。
经验证，该抗硫辛酸兔pAb可用于ELISA、冰冻切片、免疫印迹、石蜡切片检测硫辛酸。
识别天然硫辛酸。不识别被4-HNE（4-羟基-2-壬醛）和其他脂质过氧化产物修饰的硫辛酸。
Immunogen
与KLH结合的硫辛酸
Application
ELISA（1:500-1:1000）
冰冻切片（1:50-1:500）
免疫印迹（1:1000-1:10,000）
石蜡切片（1:50-1:500）
冰冻切片（1:50-1:500）
免疫印迹（1:1000-1:10,000）
石蜡切片（1:50-1:500）
Physical form
在缓冲液，50%甘油，pH 7.5中稀释的血清。
Other Notes
Sasaki, M., et al. 2000.J. Autoimmun.15, 51.
Heitzer, T., et al. 2001.Free Radic.Biol. Med.31, 53.
Patrick.L. 2000.Altern.Med. Rev.5, 290.
Shi, S.S., et al. 1999.Antioxid.Redox Signal1, 123.
Humphries, K.M. and Szeda, L.I.1998.Biochemistry37, 15835.
Heitzer, T., et al. 2001.Free Radic.Biol. Med.31, 53.
Patrick.L. 2000.Altern.Med. Rev.5, 290.
Shi, S.S., et al. 1999.Antioxid.Redox Signal1, 123.
Humphries, K.M. and Szeda, L.I.1998.Biochemistry37, 15835.
不结合被4-HNE（4-羟基-2-壬醛）和其他脂质过氧化产物修饰的硫辛酸。可用于评估蛋白质结合的硫辛酸是否已经历某些形式的氧化修饰。蛋白结合硫辛酸的存在可以作为线粒体的标志物。合适的工作稀释度由与测定条件相关的变量决定。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：标准处理（A）
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存储类别
10 - Combustible liquids
wgk
WGK 1
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Friedreich ataxia (FA) is a cardioneurodegenerative disease caused by deficient frataxin expression. This mitochondrial protein has been related to iron homeostasis, energy metabolism, and oxidative stress. Previously, we set up a cardiac cellular model of FA based on neonatal rat
Yvonne Sun et al.
Infection and immunity, 78(11), 4667-4673 (2010-09-09)
Anteiso-branched-chain fatty acids (BCFA) represent the dominant group of membrane fatty acids and have been established as crucial determinants in resistance against environmental stresses in Listeria monocytogenes, a facultative intracellular pathogen. Here, we investigate the role of anteiso-BCFA in L.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 437695-100UL | 07790788059934 |