Merck
CN
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537059

Sigma-Aldrich

碘化丙啶

Membrane impermeable DNA intercalator.

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别名:
碘化丙啶
Empirical Formula (Hill Notation):
C27H34I2N4
CAS号:
分子量:
668.39
MDL编号:

质量水平

形式

solid

manufacturer/tradename

Calbiochem®

储存条件

OK to freeze
protect from light

颜色

dark red

input

sample type intact cells

荧光

λex ~536 nm
λem ~617 nm

运输

ambient

储存温度

2-8°C

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

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此商品
81845P4170P4864
Propidium Iodide Membrane impermeable DNA intercalator.

Sigma-Aldrich

537059

碘化丙啶

Propidium iodide ≥94% (HPLC)

Sigma-Aldrich

81845

碘化丙啶

Propidium iodide ≥94.0% (HPLC)

Sigma-Aldrich

P4170

碘化丙啶

Propidium iodide solution solution (1.0 mg/ml in water)

Sigma-Aldrich

P4864

碘化丙啶 溶液

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

storage condition

OK to freeze, protect from light

storage condition

-

storage condition

-

storage condition

-

color

dark red

color

-

color

-

color

-

shipped in

ambient

shipped in

-

shipped in

-

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

一般描述

不渗透膜的DNA嵌入剂在488 nm处有红色荧光。可用于流式细胞仪染色凋亡细胞和细胞核。可用于区分凋亡和坏死细胞死亡。可与标记有异硫氰酸荧光素的普通绿色荧光探针一起使用。具有以下光谱特性:消光系数:5,900 M-1cm-1 (493 nm);55,700 M-1cm-1 (287 nm);13,800 M-1cm-1 (252.5 nm);最大发射波长:536 nm;最大发射波长:617 nm。
不渗透膜的DNA嵌入剂在488 nm处有红色荧光。适用于流式细胞仪,染色凋亡细胞和细胞核。可用于区分凋亡和坏死细胞死亡,而仅染色坏死细胞。可与用异硫氰酸荧光素(FITC)标记的普通绿色荧光探针一起使用。
消光系数(493 nm):5900 M -1cm-1;(287 nm):55,700 M -1cm-1

生化/生理作用

主靶
不渗透膜的DNA嵌入剂
产物不与ATP竞争。
可逆:否
细胞可渗透性:否

警告

毒性:刺激性(B)

制备说明

首先溶解在非常少量的DMSO中,然后向水溶液中添加材料,可以改善溶解度。

重悬

复溶后,等分并冷冻保存(-20°C)。贮备溶液在-20°C下可稳定保存至多6个月。

其他说明

Belloc, F., et al. 1994.Cytometry 17, 59.
Crompton, T., et al. 1992.Biochem.Biophys.Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992.Cytometry13, 795.
de Caestecker, M.P., et al. 1992.J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992.Cytometry13, 432.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Health hazard

警示用语:

Warning

危险声明

危险分类

Muta. 2

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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