537059
碘化丙啶
Membrane impermeable DNA intercalator.
别名:
碘化丙啶
质量水平
描述
RTECS - SF7949600
表单
solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
protect from light
颜色
dark red
输入
sample type intact cells
荧光
λex ~536 nm
λem ~617 nm
运输
ambient
储存温度
2-8°C
SMILES字符串
[I-].I.[N+](CCCN1C2=CC(=N)C=CC2=C3C(=C1c4ccccc4)C=C(C=C3)N)(CC)(CC)C
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI key
XJMOSONTPMZWPB-UHFFFAOYSA-M
相关类别
一般描述
不渗透膜的DNA嵌入剂在488 nm处有红色荧光。可用于流式细胞仪染色凋亡细胞和细胞核。可用于区分凋亡和坏死细胞死亡。可与标记有异硫氰酸荧光素的普通绿色荧光探针一起使用。具有以下光谱特性：消光系数：5,900 M-1cm-1 (493 nm)；55,700 M-1cm-1 (287 nm)；13,800 M-1cm-1 (252.5 nm)；最大发射波长：536 nm；最大发射波长：617 nm。
消光系数（493 nm）：5900 M -1cm-1；(287 nm)：55,700 M -1cm-1；
生化/生理作用
主靶
不渗透膜的DNA嵌入剂
不渗透膜的DNA嵌入剂
产物不与ATP竞争。
可逆：否
细胞可渗透性：否
制备说明
复溶后，等分并冷冻保存（-20°C）。贮备溶液在-20°C下可稳定保存至多6个月。
首先溶解在非常少量的DMSO中，然后向水溶液中添加材料，可以改善溶解度。
其他说明
Belloc, F., et al. 1994.Cytometry 17, 59.
Crompton, T., et al. 1992.Biochem.Biophys.Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992.Cytometry13, 795.
de Caestecker, M.P., et al. 1992.J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992.Cytometry13, 432.
Crompton, T., et al. 1992.Biochem.Biophys.Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992.Cytometry13, 795.
de Caestecker, M.P., et al. 1992.J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992.Cytometry13, 432.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
毒性：刺激性（B）
警示用语：
Warning
危险声明
危险分类
Muta. 2
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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