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Merck
CN

537060

Propidium Iodide Solution

A convenient form of propidium iodide useful for flow cytometry studies.

别名:

Propidium Iodide Solution

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关于此项目

经验公式（希尔记法）:
C27H34I2N4
化学文摘社编号:
25535-16-4
分子量:
668.39
UNSPSC Code:
12352200
NACRES:
NA.47
MDL number:
MFCD00011921

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SMILES string

[I-].I.[N+](CCCN1C2=CC(=N)C=CC2=C3C(=C1c4ccccc4)C=C(C=C3)N)(CC)(CC)C

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

description

RTECS - SF7949600

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

input

sample type intact cells

fluorescence

λex 536 nm, λem 617 nm

shipped in

ambient

storage temp.

2-8°C

Quality Level

100

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相关类别

General description

Membrane impermeant DNA intercalator. Useful for staining apoptotic cells and nuclei by flow cytometry. Useful in differentiating between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes, e.g. fluorescein isothiocyanate. Excitation max.: ~536 nm; Emission max.: ~617 nm.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Membrane impermeable DNA intercalator
Product does not compete with ATP.
Reversible: no

Physical form

50 µg/ml in PBS, pH 7.4.

Preparation Note

Following initial use, aliquot and freeze (-20°C).

Other Notes

Belloc, F., et al. 1994. Cytometry17, 59.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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实验方案

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