Product Name
蛋白酶抑制剂混合物套装I, A cocktail of five protease inhibitors that will inhibit a broad range of proteases and esterases. Supplied with a data sheet.
表单
lyophilized
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
desiccated (hygroscopic)
溶解性
water: soluble
运输
wet ice
储存温度
−20°C
一般描述
蛋白酶抑制剂混合液Set I A 是一种专门配制的混合液，含有可抑制各种蛋白酶和酯酶的五种不同蛋白酶抑制剂。
应用
蛋白酶抑制剂混合液 Set I – Calbiochem 已用于裂解缓冲液中以裂解细胞并进行蛋白免疫印迹。
蛋白酶抑制剂鸡尾酒套装I已用作以下产品的补充剂：
- 冷放射免疫沉淀法（RIPA）缓冲液，用于制备蛋白质印迹分析中的细胞裂解液
- RIPA裂解缓冲液，用于提取大鼠肝组织总蛋白和AML12细胞
- 蔗糖溶液，用于均质化小鼠皮质以分离髓鞘
生化/生理作用
抑制多种蛋白酶和酯酶。
蛋白酶抑制剂鸡尾酒和特异性蛋白酶抑制剂是科学家在各种蛋白质组学研究中使用的重要工具。这些研究包括蛋白质表达、表征、生物标志物发现、翻译后修饰、蛋白质表达谱和定量蛋白质测量。蛋白酶抑制剂鸡尾酒或蛋白酶抑制剂的应用保证了蛋白质完整性的保存，便于下游分析和表征。
特点和优势
- 蛋白酶抑制剂混合液保护粗提取物中的目标蛋白免于分解
- 可立即用于重构（reconstitution）
制备说明
复溶后，等分并冷冻保存（-20°C）。贮备溶液在-20°C下可稳定保存至多1个月。
用1 ml dH2O复溶每个小瓶，以获得100X的贮备溶液。
其他说明
如果直接用IMAC树脂处理细胞提取物，EDTA 的存在可能干扰His•Tag 融合蛋白的纯化。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
毒性：有害（C）
警示用语：
Danger
危险声明
危险分类
Acute Tox. 4 Inhalation - Eye Dam. 1 - Skin Corr. 1A - STOT RE 2 Inhalation
靶器官
Respiratory Tract
储存分类代码
8B - Non-combustible corrosive hazardous materials
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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