574777
兔抗突触素I多克隆抗体
lyophilized, Calbiochem®
别名:
兔抗突触素I抗体, 突触素I检测抗体
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human, rat, mouse, bovine
Application:
—
Citations:
20
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
species reactivity
human, rat, mouse, bovine
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(ELISA (1:2500-1:10,000)
Frozen Sections
Immunoblotting (1:200)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (1 µg/200 µg lysate))
isotype
IgG
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Syn1(20964)
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General description
免疫亲和纯化的兔多克隆抗体。识别突触I蛋白。
识别大鼠大脑中的突触I蛋白。
这种抗突触蛋白I兔pAb经验证可用于 ELISA、免疫印迹、免疫细胞化学、免疫沉淀，以检测突触蛋白I。
Immunogen
牛
纯化的牛脑突触素I
Application
ELISA（1:2500-1:10000）
冰冻切片（见应用参考文献）
免疫印迹（1:200）
免疫细胞化学（1:500-1:2500）
免疫沉淀（1 µg/200 µg裂解物）
冰冻切片（见应用参考文献）
免疫印迹（1:200）
免疫细胞化学（1:500-1:2500）
免疫沉淀（1 µg/200 µg裂解物）
Physical form
从5 mM碳酸氢铵中冻干。
Preparation Note
用50µl PBS复溶。复溶后，等分并冷冻保存（-20°C）。避免溶液反复冻融。
Analysis Note
大鼠脑
阳性对照
阳性对照
Other Notes
Stone, L.M., et al. 1994.J. Neurosci.14, 301.
Mandell, J.W., et al. 1992.J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991.J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989.J. Neurosci.9, 2151.
Mandell, J.W., et al. 1992.J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991.J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989.J. Neurosci.9, 2151.
该抗体对突触蛋白I具有特异性，可用于估计突触密度和突触发生。在1:200稀释的免疫印迹中可以看到突触蛋白I双峰的强而特异的免疫标记。这种免疫标记可以通过抗体与突触蛋白I的预吸附来阻止。与测定条件相关的变量将决定适当的工作稀释度。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：标准处理（A）
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存储类别
11 - Combustible Solids
wgk
WGK 1
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