生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
lyophilized
不包含
preservative
种属反应性
human, rat, mouse, bovine
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
dilution
(ELISA (1:2500-1:10,000)
Frozen Sections
Immunoblotting (1:200)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (1 µg/200 µg lysate))
同位素/亚型
IgG
运输
ambient
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
mouse ... Syn1(20964)
一般描述
免疫亲和纯化的兔多克隆抗体。识别突触I蛋白。
识别大鼠大脑中的突触I蛋白。
这种抗突触蛋白I兔pAb经验证可用于 ELISA、免疫印迹、免疫细胞化学、免疫沉淀，以检测突触蛋白I。
免疫原
牛
纯化的牛脑突触素I
应用
ELISA（1:2500-1:10000）
冰冻切片（见应用参考文献）
免疫印迹（1:200）
免疫细胞化学（1:500-1:2500）
免疫沉淀（1 µg/200 µg裂解物）
冰冻切片（见应用参考文献）
免疫印迹（1:200）
免疫细胞化学（1:500-1:2500）
免疫沉淀（1 µg/200 µg裂解物）
外形
从5 mM碳酸氢铵中冻干。
制备说明
用50µl PBS复溶。复溶后，等分并冷冻保存（-20°C）。避免溶液反复冻融。
分析说明
大鼠脑
阳性对照
阳性对照
其他说明
Stone, L.M., et al. 1994.J. Neurosci.14, 301.
Mandell, J.W., et al. 1992.J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991.J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989.J. Neurosci.9, 2151.
Mandell, J.W., et al. 1992.J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991.J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989.J. Neurosci.9, 2151.
该抗体对突触蛋白I具有特异性，可用于估计突触密度和突触发生。在1:200稀释的免疫印迹中可以看到突触蛋白I双峰的强而特异的免疫标记。这种免疫标记可以通过抗体与突触蛋白I的预吸附来阻止。与测定条件相关的变量将决定适当的工作稀释度。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
毒性：标准处理（A）
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储存分类代码
11 - Combustible Solids
WGK
WGK 1
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