产品名称
Rosetta-gami B(DE3)pLysS 感受态细胞 - Novagen, Rosetta-gami B strains combines the key features of BL21, Origami, and Rosetta to enhances the expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.
biological source
Escherichia coli
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
transformation efficiency: 1.0×106 cfu/μg
shipped in
dry ice
storage temp.
−70°C
General description
Rosetta-gami B菌株结合了BL21(及其Tuner衍生物)、Origami和Rosetta的关键特征,以增强真核蛋白的表达和细菌细胞质中靶蛋白二硫键的形成。这些菌株与抗氨苄青霉素或大观霉素的载体相容。
DE3表明宿主是λDE3的溶原菌,因此在lacUV5启动子的控制下携带T7 RNA聚合酶基因的染色体拷贝。此类菌株适用于通过IPTG诱导从克隆到pET载体中的靶基因生产蛋白质。
pLysS菌株表达T7溶菌酶,其在诱导前进一步抑制T7 RNA聚合酶的基础表达,从而稳定编码影响细胞生长和活力的靶蛋白的pET重组体。
DE3表明宿主是λDE3的溶原菌,因此在lacUV5启动子的控制下携带T7 RNA聚合酶基因的染色体拷贝。此类菌株适用于通过IPTG诱导从克隆到pET载体中的靶基因生产蛋白质。
pLysS菌株表达T7溶菌酶,其在诱导前进一步抑制T7 RNA聚合酶的基础表达,从而稳定编码影响细胞生长和活力的靶蛋白的pET重组体。
基因型:F–ompT hsdSB (rB– mB–) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pLysSRARE (CamR, KanR, TetR)
该产品包含转基因生物(GMO)。在欧盟范围内,转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击这里获得最终用户声明(EUD)表格。
该产品包含转基因生物(GMO)。在欧盟范围内,转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。该法规规定我们有义务索取您以及处理转基因生物的机构的相关信息。请点击这里获得最终用户声明(EUD)表格。
Rosetta-gami B菌株结合了BL21、Origami和Rosetta的关键特征,以增强真核蛋白的表达。T7溶菌酶表达抑制基础T7表达。
Other Notes
0.4 ml1 ml组分
•2 × 0.2 ml5 × 0.2 mlRosetta-gami B(DE3)pLysS感受态细胞
•2 × 2 ml4 × 2 mlSOC培养基
•10 µl10 µl供试质粒
•2 × 0.2 ml5 × 0.2 mlRosetta-gami B(DE3)pLysS感受态细胞
•2 × 2 ml4 × 2 mlSOC培养基
•10 µl10 µl供试质粒
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性:多个毒性值,请参阅MSDS(O)
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 71137-3 | 07790788053079 |
| 71137-4 | 04055977254761 |