Rosetta-gami B(DE3)pLysS 感受态细胞 - Novagen

Rosetta-gami B菌株结合了BL21（及其Tuner衍生物）、Origami和Rosetta的关键特征，以增强真核蛋白的表达和细菌细胞质中靶蛋白二硫键的形成。这些菌株与抗氨苄青霉素或大观霉素的载体相容。

DE3表明宿主是λDE3的溶原菌，因此在lacUV5启动子的控制下携带T7 RNA聚合酶基因的染色体拷贝。此类菌株适用于通过IPTG诱导从克隆到pET载体中的靶基因生产蛋白质。

pLysS菌株表达T7溶菌酶，其在诱导前进一步抑制T7 RNA聚合酶的基础表达，从而稳定编码影响细胞生长和活力的靶蛋白的pET重组体。

基因型：F^–ompT hsdS_B (r_B^– m_B^–) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pLysSRARE (Cam^R, Kan^R, Tet^R)

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Rosetta-gami B菌株结合了BL21、Origami和Rosetta的关键特征，以增强真核蛋白的表达。T7溶菌酶表达抑制基础T7表达。

0.4 ml1 ml组分

•2 × 0.2 ml5 × 0.2 mlRosetta-gami B(DE3)pLysS感受态细胞

•2 × 2 ml4 × 2 mlSOC培养基

•10 µl10 µl供试质粒

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毒性：多个毒性值，请参阅MSDS（O）