AB1431
抗干扰素-β 抗体
serum, Chemicon®
别名:
IFN-beta
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA
immunohistochemistry
immunoprecipitation (IP)
neutralization
western blot
immunohistochemistry
immunoprecipitation (IP)
neutralization
western blot
Species reactivity:
human
Citations:
12
Technique(s):
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
western blot: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗干扰素-β 抗体, serum, Chemicon®
Quality Level
biological source
rabbit
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... IFNB1(3456)
Analysis Note
对照
IFN表达细胞系
IFN表达细胞系
Application
干扰素中和：2 x 10E5中和单位/mL，其中一个中和单位是将一个单位的人干扰素β（Hu-IFN-b）中和至50%终点所需的抗血清量。使用如[Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," in J. Virol., Vol. 37, 755-758]；[Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods of Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]中所述的细胞病变效应抑制测定法滴定干扰素。在该干扰素的抗病毒测定中，约1单位/毫升的干扰素是产生50%的细胞病变效应所需的量。单位是根据美国国立卫生研究院提供的人干扰素β的国际标准品确定的[参见Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]。该材料是专门为有效中和人干扰素β而制备的。
免疫组化
蛋白质印迹：1:5000
免疫沉淀
ELISA
最佳工作稀释度必须由最终用户进行确定。
免疫组化
蛋白质印迹：1:5000
免疫沉淀
ELISA
最佳工作稀释度必须由最终用户进行确定。
研究子类别
细胞因子&细胞因子受体
细胞因子&细胞因子受体
研究类别
炎症 & 免疫学
炎症 & 免疫学
该抗干扰素-β抗体经验证可用于ELISA、IH、IP、NEUT、WB中检测干扰素-β。
Biochem/physiol Actions
识别人干扰素β。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
20 kDa
干扰素β是一种球状蛋白，由5个α螺旋组成，预测分子量为20 kDa。然而，由于糖基化，通过SDS-PAGE观察到的分子量接近25 kDa。
Immunogen
在大肠埃希菌中表达的重组人干扰素β。
Other Notes
替代品：04-1095
Physical form
未纯化
用PBS稀释的兔血清。 不含防腐剂的液体。
Preparation Note
自收到之日起在-20°C可稳定保存1年。避免反复冻融。为了最大程度地回收产品，在融化后和取下盖子之前，将原始小瓶进行离心。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
You-Ping Deng et al.
Experimental and therapeutic medicine, 14(1), 753-758 (2017-07-05)
Human bocavirus (HBoV) is classified in the Bocavirus genus within the Parvoviridae family, first identified from children with respiratory diseases. Previous studies have investigated the stimulating effect of HBoV on cell apoptosis and autophagy. In the present study, human bronchial
A hyperfusogenic F protein enhances the oncolytic potency of a paramyxovirus simian virus 5 P/V mutant without compromising sensitivity to type I interferon.
Gainey, MD; Manuse, MJ; Parks, GD
Journal of virology null
Jincheng Chen et al.
Microbiology spectrum, 9(2), e0047321-e0047321 (2021-10-21)
The aryl hydrocarbon receptor (AHR) is a ligand-activated transcript factor that plays an important role in regulating immunity and cell differentiation. However, its role in cell-autonomous antiviral resistance has not been fully elucidated. Here, we show that interruption of AHR
Viacheslav Malikov et al.
Cell reports, 38(7), 110396-110396 (2022-02-17)
Fasciculation and elongation protein zeta-1 (FEZ1) is a multifunctional kinesin adaptor involved in processes ranging from neurodegeneration to retrovirus and polyomavirus infection. Here, we show that, although modulating FEZ1 expression also impacts infection by large DNA viruses in human microglia
Harumasa Nakazawa et al.
PloS one, 12(1), e0170391-e0170391 (2017-01-19)
Inflammation and apoptosis develop in skeletal muscle after major trauma, including burn injury, and play a pivotal role in insulin resistance and muscle wasting. We and others have shown that inducible nitric oxide synthase (iNOS), a major mediator of inflammation
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