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Merck
CN

AB1513P

抗脑源性神经营养因子抗体

Chemicon®, from sheep

别名:

BDNF

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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产品名称

抗脑源性神经营养因子抗体, Chemicon®, from sheep

biological source

sheep

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat, chicken

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_001709.3
NM_170731.3
NM_170732.3
NM_170733.2
NM_170734.2
NM_170735.4

UniProt accession no.

P23560

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... BDNF(627)

相关类别

Analysis Note

对照
脑组织

Application

免疫组化：1-5 μg/mL（请参阅建议的方案）。

免疫印迹：1-5 μg/mL，由于BDNF不容易在标准缓冲液中提取，我们建议用Semba-Katoh, R等人（J. Neurochem. 69(1):34- 42,1997）的方法制备用于蛋白质印迹的BDNF样品。切开的组织应在10倍体积的100mM磷酸盐缓冲液中匀浆，该缓冲液包含1mM EDTA，2M盐酸胍（pH7.2）和三种蛋白酶抑制剂，10mM N-乙基马来酰亚胺，0.36 mM胃抑素和1mM PMSF。然后对匀浆进行超声处理，并在4°C下以46K x g离心30分钟。 BDNF以~12-18kDa的单体迁移，在大多数样品中具有~12-18kDa二聚体。

ELISA：1-5 μg/mL

体外生物活性抑制：在1-5 μg/mL浓度下，BDNF对鸡胚感觉神经元的活性被完全抑制。 生物抑制方案可参考Finn等人（J. Neurocytol.15:169-176, 1986）。
生物活性：中和BDNF，但不中和其他神经营养因子。

仅用于体内研究。

最佳工作稀释度必须由最终用户进行确定。
抗脑源性神经营养因子抗体可检测脑源性神经营养因子水平，已发布，经验证可用于ELISA、IH、FUNC & WB。
研究子类别
神经化学&神经营养因子

神经炎症&疼痛
研究类别
神经科学

Biochem/physiol Actions

可识别脑源性神经营养因子（BDNF）。中和BDNF，但不中和其他神经营养因子。通过单位点ELISA，与小鼠NGF、重组人NT3或神经营养蛋白4的交叉反应性小于1%。该抗体在人类和大鼠衍生样本上进行了测试，但基于序列相似性，预计将与小鼠和其他哺乳动物物种发生反应。 人、大鼠和小鼠BDNF的肽序列相同。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

~12-18kDa单体，大多数样品中有~12-18kDa二聚体。

Immunogen

重组人脑源性神经营养因子（BDNF）。

Other Notes

浓度：请参考批次特异性浓缩物的检验报告。

Physical form

形式：纯化
蛋白G纯化
蛋白G纯化免疫球蛋白。 从PBS中冻干，不含防腐剂。用500 μL无菌蒸馏水复溶。

Preparation Note

自收到之日起，冻干材料在-20至-70°C下可保存长达12个月。复溶后以未稀释等分试样在 -20°C保存长达 6个月。可以添加甘油（1：1）以获得额外的稳定性。应避免反复冻/融循环。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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SHEEP ANTI-BRAIN DERIVED NEUROTROPHIC FACTOR (IgG Fraction) POLYCLONAL ANTIBODY
An Introduction to Antibodies and Their Applications

The 3rd edition of An Introduction to Antibodies and Their Applications provides a concise overview of some of the key features for the use of antibodies and immunochemical techniques in biological research. This handy reference guide supplements the techniques described in literature, recorded in general laboratory procedures, and described on individual product data sheets. Antibody design, development, and production are our expertise. Stringent validation of our antibodies is only one component of a comprehensive process we undertake to provide the antibodies most cited by the research community (see section Antibody Quality on page 2 for an in-depth look at our expertise).

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