biological source
rabbit
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Immunogen
FMRFamide
Application
免疫组化：1：1,000- 1：5,000
免疫组化应用说明：
- 建议灌注步骤：
1）不含Ca2+的台氏液，
2）多聚甲醛-苦味酸固定剂
3）10%蔗糖的PBS溶液作为冷冻保护剂
-快速解剖组织并在含10%蔗糖的0.1 M磷酸盐缓冲液（pH 7.4）中保存过夜。
-将载玻片固定的组织切片与封闭缓冲液在室温下培养1小时。
- 去除封闭缓冲液，并在2-8°C下将载玻片和一抗（将封闭缓冲液中的AB15348在稀释至需要的工作浓度）一起培养18-24小时。
-去除一抗，并以PBS冲洗3次。
- 将切片与荧光标记的二抗在室温下培养60分钟。
最佳工作稀释度必须由最终用户进行确定。
免疫组化应用说明：
- 建议灌注步骤：
1）不含Ca2+的台氏液，
2）多聚甲醛-苦味酸固定剂
3）10%蔗糖的PBS溶液作为冷冻保护剂
-快速解剖组织并在含10%蔗糖的0.1 M磷酸盐缓冲液（pH 7.4）中保存过夜。
-将载玻片固定的组织切片与封闭缓冲液在室温下培养1小时。
- 去除封闭缓冲液，并在2-8°C下将载玻片和一抗（将封闭缓冲液中的AB15348在稀释至需要的工作浓度）一起培养18-24小时。
-去除一抗，并以PBS冲洗3次。
- 将切片与荧光标记的二抗在室温下培养60分钟。
最佳工作稀释度必须由最终用户进行确定。
抗-FMRFamid 抗体是针对FMRFamide的抗体，可用于IH。
研究子类别
激素 & 受体
激素 & 受体
研究类别
神经科学
神经科学
Physical form
含0.05%叠氮化钠的液体。
血清
Preparation Note
自收到之日起，在 -20°C 以未稀释的等分试样可保存 6 个月。应避免反复冻/融循环。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 1
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Biology, 9(9) (2020-09-10)
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Frontiers in neuroscience, 17, 1125476-1125476 (2023-06-19)
Cilia are the major effectors in Ctenophores, but very little is known about their transmitter control and integration. Here, we present a simple protocol to monitor and quantify cilia activity and provide evidence for polysynaptic control of cilia coordination in
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Anatomical record (Hoboken, N.J. : 2007), 301(6), 1103-1114 (2018-01-19)
Neuropeptides AF (NPAF), FF (NPFF) and SF (NPSF) are RFamide neuropeptides known to be widely expressed in the mammalian central nervous system, where they fulfill a wide range of functions with pain modulation being the most prominent one. Recent evidence
Daniel Kueh et al.
The Journal of comparative neurology, 521(12), 2833-2849 (2013-02-26)
The formation and refinement of synaptic connections are dependent on the activity that emerges from nascent synaptic connections. Such activity has the effect of regulating the production and release of specific neurotransmitters. To determine the role of activity in regulating
An Inhibitory Circuit From Brainstem to GnRH Neurons in Male Mice: A New Role for the RFRP Receptor.
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Endocrinology, 162(5) (2021-02-11)
RFamide-related peptides (RFRPs, mammalian orthologs of gonadotropin-inhibitory hormone) convey circadian, seasonal, and social cues to the reproductive system. They regulate gonadotropin secretion by modulating gonadotropin-releasing hormone (GnRH) neurons via the RFRP receptor. Mice lacking this receptor are fertile but exhibit
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