AB1558
抗多巴胺D2受体抗体
serum, Chemicon®
别名:
Anti-D2DR, Anti-D2R
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA
immunocytochemistry
immunohistochemistry
western blot
immunocytochemistry
immunohistochemistry
western blot
Species reactivity:
rat
Citations:
22
Technique(s):
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗多巴胺D2受体抗体, serum, Chemicon®
biological source
rabbit
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
rat ... Drd2(24318)
Analysis Note
对照
脑
脑
Application
免疫组化：4%PFA固定，冰冻切片，1:200，室温孵育24小时，DAB检测。{Tillerson, JL et al. (2006) Behavioural Brain Res. 172:97-105.
免疫细胞化学：4% PFA固定细胞10-15′，用0.2-0.3%triton X-100透化，1:100，O/N 4c用于荧光 {Sun, W et al (2005) Stem Cells 23(7):931-945, http://stemcells.alphamedpress.org/cgi/content/full/23/7/931}。
蛋白质印迹：1:1,000-1:5,000（化学发光技术）当使用低浓度样品或在抗体稀释剂中使用牛奶时，可能需要更高浓度的抗体。可能需要加载100-200 μg纯化的纹状体或其他组织膜以可视化DR2条带。已显示该抗体可识别天然和变性DR2条带。
ELISA：1:10,000-1:50,000 [1 μg/mL D2R肽（目录编号AG284）/孔]
最佳工作稀释度必须由最终用户进行确定。
抗多巴胺D2受体抗体是用于ELISA、IC、IH & WB的抗多巴胺D2受体抗体。
研究子类别
神经递质& 受体
神经递质& 受体
研究类别
神经科学
神经科学
Biochem/physiol Actions
识别多巴胺D2受体（D2R）。识别天然和变性形式的D2R（使用大鼠纹状体阳性对照（目录号AG282），通过蛋白质印迹法检测110、68和47 kDa条带）。AB1558抑制[3H]YM-09152-2（一种已知的D2R拮抗剂）和（125）I-NAPS（一种光配体）的结合。它抑制[3H]YM-09152-2和PPHT-荧光素（D2R的激动剂）的结合，并与转染了大鼠D2R cDNA的成纤维细胞中的110 kDa D2R条带反应。AG284可用于封闭实验；不建议将AG284用作蛋白质印迹的阳性对照。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
110, 68和47 kDa
Immunogen
大鼠D2R（1，2）的配体结合域或附近的氨基末端肽序列（aa 24-34）。
免疫原与小鼠同源性为100%，与人和牛同源性为90%，与犬D2R受体同源性为72%。针对相同序列制成的抗体已显示出与人、龙虾、鱿鱼和小龙虾的反应性。
免疫原与小鼠同源性为100%，与人和牛同源性为90%，与犬D2R受体同源性为72%。针对相同序列制成的抗体已显示出与人、龙虾、鱿鱼和小龙虾的反应性。
Other Notes
浓度：请参考批次特异性浓缩物的检验报告。
Physical form
兔抗血清。含0.05%叠氮化钠的液体。
未纯化
Preparation Note
自收到之日起在-20°C可稳定保存1年。避免反复冻融。为了最大程度地回收产品，在融化后和取下盖子之前，将原始小瓶进行离心。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
Alexandre Morin et al.
Frontiers in behavioral neuroscience, 15, 770374-770374 (2022-01-22)
Global cerebral ischemia (GCI) in rats has been shown to promote exploration of anxiogenic zones of the Elevated-Plus Maze (EPM) and Open Field Test (OFT). This study investigated changes in impulsive choice and/or defensive responses as possible contributors of heightened
K E Borgmann-Winter et al.
Neuroscience, 158(2), 642-653 (2008-11-11)
The olfactory epithelium constitutes the sole source of regenerating neural cells that can be obtained from a living human. As such, primary cultures derived from human olfactory epithelial biopsies can be utilized to study neurobiological characteristics of individuals under different
Violeta-Maria Caragea et al.
Brain structure & function, 229(3), 609-637 (2023-08-24)
The dopaminergic (DA) system regulates both motor function, and learning and memory. The cerebellum supports motor control and the acquisition of procedural memories, including goal-directed behavior, and is subjected to DA control. Its fastigial nucleus (FN) controls and interprets body
Andrea L Johnstone et al.
Molecular and cellular neurosciences, 50(2), 125-135 (2012-05-09)
Injury to the central nervous system (CNS) can result in lifelong loss of function due in part to the regenerative failure of CNS neurons. Inhibitory proteins derived from myelin and the astroglial scar are major barriers for the successful regeneration
Hui Leng et al.
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 31(9), e13655-e13655 (2019-06-07)
Oxytocin (OT) plays an important role in regulating gastric function. How OT regulates stress-induced gastric ulcers is not understood. We investigated OT's protective role in stress-induced gastric ulcers, with a focus on OT's interaction with the ventral tegmental area (VTA)
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