产品名称
抗腺苷A1受体抗体, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, rat, sheep, guinea pig, pig
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ADORA1(134)
Analysis Note
对照
脑组织
脑组织
Application
免疫组化:10-50 μg/mL,在冷冻组织切片上。建议光固定。2% 0.2%苦味酸 {Cooke, HJ (1999) AJP Gastrointest Liver Physiol 276:4516-4626}
免疫细胞化学:Caufield, 2003。轻度2%PFA固定5-10分钟-长时间固定降低染色强度。
蛋白质印迹:1-10 μg/mL(化学发光技术):建议膜制备用于增强信号{Smith et al (2001) Biochem Pharmacology 61:237-244} 如果使用量热检测或基于牛奶的抗体稀释剂,则可能需要更高浓度的抗体。
ELISA:1:10,000-1:50,000(50-100 ng A1R肽(目录编号AG289)/孔)
最佳工作稀释度必须由最终用户进行确定。
免疫细胞化学:Caufield, 2003。轻度2%PFA固定5-10分钟-长时间固定降低染色强度。
蛋白质印迹:1-10 μg/mL(化学发光技术):建议膜制备用于增强信号{Smith et al (2001) Biochem Pharmacology 61:237-244} 如果使用量热检测或基于牛奶的抗体稀释剂,则可能需要更高浓度的抗体。
ELISA:1:10,000-1:50,000(50-100 ng A1R肽(目录编号AG289)/孔)
最佳工作稀释度必须由最终用户进行确定。
抗腺苷A1受体抗体检测腺苷A1受体的水平 & 经发布 & 经验证可用于ELISA、IC、IH & WB。
研究子类别
神经递质 & 受体
昼夜节律 & 睡眠
神经递质 & 受体
昼夜节律 & 睡眠
研究类别
神经科学
神经科学
Biochem/physiol Actions
可识别腺苷A1受体(A1R)。识别来自大鼠、猪和羔羊的皮质膜中的79 kDa和39 kDa条带。79 kDa的条带可以通过激动剂或拮抗剂处理转化为39 kDa的条带,并且可以代表A1R二聚体。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
79 kDa和39 kDa
Immunogen
对应于大鼠A1R基因的第三个细胞外结构域(氨基酸163-176,GEPVIKCEFEKVIS)的氨基酸序列。
Other Notes
浓度:请参考批次特异性浓缩物的检验报告。
Physical form
亲和纯化的免疫球蛋白。 含0.1% BSA的PBS溶液。
免疫亲和纯化
Preparation Note
自收到之日起在-20°C可稳定保存1年。避免反复冻融。为了最大程度地回收产品,在融化后和取下盖子之前,将原始小瓶进行离心。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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