产品名称
抗连接蛋白40抗体, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GJA5(2702)
Analysis Note
对照
胎盘裂解液。
胎盘裂解液。
已在Jurkat裂解液中通过蛋白质印迹进行了评估。
蛋白质印迹分析：该批次的产品已以1：1000的稀释度在10 μg Jurkat裂解液中检测到连接蛋白40。
蛋白质印迹分析：该批次的产品已以1：1000的稀释度在10 μg Jurkat裂解液中检测到连接蛋白40。
Application
免疫组化分析：代表性批次的1:50稀释液检测到与小鼠心脏组织切片中的心肌上皮细胞之间的间隙连接相关的连接蛋白40免疫反应性。
蛋白质印迹分析：代表性批次的1:1000稀释液在人（HEK293，HeLa，HepG2，HUVEC，Jurkat）和鼠（C2C12，HIN/3T3）细胞裂解液中检测到连接蛋白40。
蛋白质印迹分析：代表性批次的1:000稀释液在人、小鼠和大鼠心脏组织匀浆以及小鼠脑组织匀浆中检测到连接蛋白40。
最佳工作稀释度必须由最终用户确定。
蛋白质印迹分析：代表性批次的1:1000稀释液在人（HEK293，HeLa，HepG2，HUVEC，Jurkat）和鼠（C2C12，HIN/3T3）细胞裂解液中检测到连接蛋白40。
蛋白质印迹分析：代表性批次的1:000稀释液在人、小鼠和大鼠心脏组织匀浆以及小鼠脑组织匀浆中检测到连接蛋白40。
最佳工作稀释度必须由最终用户确定。
抗连接蛋白40抗体是用于IHC & WB的抗Cx40抗体。
Biochem/physiol Actions
与其他物种的反应性尚未确定。
可与小鼠连接蛋白40反应。
General description
40 kDa
小鼠连接蛋白40是一种含有358个氨基酸的间隙连接蛋白，预测的分子量约为40 kDa。它在肺、心脏和皮肤中显著表达（见综述：Kumar & Giula 1996; White, 1995; Evans, 1994; Beyer , 1990; Henneman, 1992; Traub, 1994; Goliger & Paul 1994）。一个间隙连接由紧密堆积的跨膜通道对簇组成，即连接子，低分子量的材料通过该通道从一个细胞扩散到相邻细胞。连接子由连接蛋白的六聚体组成。
Immunogen
抗连接蛋白40是通过靶向小鼠C×40（2）细胞质结构域内的C端19个氨基酸的肽序列获得的。肽序列在人和大鼠Cx40（100%），鸡Cx42（77%），人Cx50（87%），大鼠Cx50（81%）和绵羊Cx49（87%）中是保守的。
Other Notes
浓度：请参考批次特异性浓缩物的检验报告。
Physical form
纯化的兔多克隆抗体，溶于含有0.1 M Tris-甘氨酸（pH 7.4）、15 mM NaCl和0.05％叠氮化钠的缓冲液中。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Markus Boettcher et al.
Hypertension (Dallas, Tex. : 1979), 57(4), 802-808 (2011-03-02)
The endothelium-derived hyperpolarizing factor (EDHF) contributes critically to the regulation of vascular tone. Its dependency on direct signaling through myoendothelial gap junctions composed of connexins (Cx) is controversially discussed. We studied the impact of Cx40 in EDHF-type dilations in vivo
Alexander Jobs et al.
Hypertension (Dallas, Tex. : 1979), 60(6), 1422-1429 (2012-10-24)
The gap junction channel protein connexin40 (Cx40) is crucial in vascular and renal physiology, because Cx40-deficient mice exhibit impaired conduction of endothelium-dependent dilations and pronounced hypertension. The latter precludes mechanistic insights into the role of endothelial Cx40, because long-lasting hypertension
Anja Hagen et al.
Cardiovascular research, 83(2), 405-415 (2009-01-30)
Ventricular arrhythmia is one of the most important causes of death in industrialized countries and often accompanies myocardial infarction and heart failure. In recent years modification of gap-junctional coupling has been proposed as a new antiarrhythmic principle. We wanted to
Volker J Schmidt et al.
PloS one, 8(11), e78782-e78782 (2013-11-16)
Gap junctions are involved in vascular growth and their expression pattern is modulated in response to hemodynamic conditions. They are clusters of intercellular channels formed by connexins (Cx) of which four subtypes are expressed in the cardiovascular system, namely Cx37
Connexin isoform expression in smooth muscle cells and endothelial cells of hamster cheek pouch arterioles and retractor feed arteries.
Hakim, CH; Jackson, WF; Segal, SS
Microcirculation (New York, N.Y. : 1994) (1994)
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