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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
human, mouse, horse, rat
Application:
western blot
Technique(s):
western blot: suitable
Citations:
251
Uniprot accession no.:
产品名称
抗GAPDH 抗体, from chicken, purified by affinity chromatography
biological source
chicken
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse, horse, rat
species reactivity (predicted by homology)
canine (based on 100% sequence homology), equine (based on 100% sequence homology)
packaging
antibody small pack of 25 μg
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GAPDH(2597)
mouse ... Gapdh(14433)
Analysis Note
对照
HEK293 细胞裂解液
HEK293 细胞裂解液
已通过蛋白质印迹在HEK293和PC12细胞裂解物中进行了评估。
蛋白质印迹分析:0.0625 µg/ml的抗体在10 µg HEK293和PC12细胞裂解物中检测到GAPDH。
蛋白质印迹分析:0.0625 µg/ml的抗体在10 µg HEK293和PC12细胞裂解物中检测到GAPDH。
Application
抗 GAPDH 抗体检测 GAPDH 的水平,&经发布 & 验证可用于 WB。
研究类别
代谢
代谢
研究类别
新陈代谢
新陈代谢
蛋白质印迹(SNAP ID)分析:0.125 µg/ml先前批次的产品在10 µg NIH/3T3和PC12细胞裂解物中检测到GAPDH。
Biochem/physiol Actions
该抗体可识别GAPDH。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
3-磷酸甘油醛脱氢酶(GAPDH)是一种普遍存在的糖酵解酶,在几乎所有组织中都以相当高的水平存在。作为一个′管家′酶,它可催化1,3-二磷酸甘油酸酯的合成,这是一种用于合成ATP的“高能”中间体。除了其在代谢中的细胞质作用外,它还参与细胞凋亡或氧化应激反应的初始阶段,在这些过程中GAPDH会易位至细胞核中。这种作用可能反映了GAPDH在DNA修复中的作用或作为凋亡分子的一种核载体。研究还发现GAPDH可特异性结合与多种神经退行性疾病的发病机制有关的蛋白质,包括β-淀粉样蛋白前体蛋白和亨廷顿蛋白,其中GAPDH功能下降与阿尔茨海默症′和亨廷顿′病成纤维细胞有关。GAPDH还被确定为一氧化氮(NO)介导的细胞毒性的潜在靶标。功能完整的酶是一个四聚体,四个相同的亚基各自占据四面体的顶点。结合结构域还包含一个主要与NAD+相互作用的结构域,另一个与3-磷酸甘油醛(GAP)相互作用的结构域。
~ 38 kDa
Immunogen
与人GAPDH的WARS相互作用区域相对应的KLH偶联肽。
与人GAPDH的WARS相互作用区域相对应的KLH缀合线性肽。
表位:WARS相互作用区域
Other Notes
浓度:请参考批次特异性浓缩物的分析证书。
浓度:请参考特定批次的数据表。
Physical form
亲和纯化
含 0.1 M Tris-甘氨酸(pH 7.4,,150 mM NaCl )和 0.05% 叠氮化钠的缓冲液中的纯化的鸡多克隆抗体。
Preparation Note
自发运之日起,在 2-8°C 条件下可稳定保存1年
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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