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Merck
CN

AB3553

抗噻嗪敏感性NaCl协同转运蛋白抗体

Chemicon®, from rabbit

别名:

Anti-NCC, Anti-NCCT, Anti-TSC

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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产品名称

抗噻嗪敏感性NaCl协同转运蛋白抗体, Chemicon®, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rabbit, mouse, rat, human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... SLC12A3(6559)

Analysis Note

对照
阳性对照: 大鼠肾。

Application

免疫印迹:使用大鼠肾提取物为1:2,000。该抗体与160-190 kDa蛋白反应(分子量变化取决于蛋白提取/电泳的条件)。建议的稀释液和封闭缓冲液为150 mM NaCl,10 mM Tris,pH值7.4,其中含有5%低脂牛奶和0.04%吐温。建议转移膜为PVDF或尼龙。建议的凝胶百分比为7%。建议在2-8°C下与抗体过夜培养。

免疫组化:在大鼠或小鼠肾组织上为1:500-1:2,000。建议的固定剂是3%多聚甲醛,通过血管灌注固定5分钟。建议的封闭缓冲液是含有10%正常山羊血清的PBS。建议的稀释缓冲液是含有1%BSA的PBS。建议在2-8°C下与抗体过夜培养。最佳工作稀释度必须由最终用户确定。
研究子类别
离子通道 & 转运体
研究类别
神经科学
该抗噻嗪敏感性NaCl协同转运蛋白抗体经验证可用于IH,WB中检测噻嗪敏感性NaCl协同转运蛋白。

Biochem/physiol Actions

识别肾噻嗪敏感性NaCl协同转运蛋白(NCC)。该抗体染色肾远曲小管细胞中的顶端质膜和顶下膜囊泡。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

160-190kda

Immunogen

来自人/大鼠/小鼠NCC N末端的合成肽。

Other Notes

替代品:AB3564P
浓度:请参考批次特异性浓缩物的分析证书。

Physical form

免疫亲和纯化
在不含防腐剂的PBS中亲和纯化的免疫球蛋白。

Preparation Note

自发运之日起在-20°C可保存1年。等分以避免反复冻融。为了最大程度地回收产品,在融化后和取下盖子之前,将原始样品瓶进行离心。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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