AB4340
抗-Zscan4抗体
from rabbit, purified by affinity chromatography
别名:
Zinc finger and SCAN domain containing protein 4C
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
26
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
General description
目标分子量：观察值为~60 kDa；计算值为60.0 kDa。在一些裂解液中可能会观察到未表征的条带。
锌指和SCAN结构域含蛋白4C（Zscan4c，也称为Gm397）基因（Gene ID: 245109）编码锌指和SCAN结构域含蛋白4C（UniProt: Q80VJ6），该基因在小鼠中表达。Zscan4是一种胚胎干细胞（ES细胞）特异性的转录因子，对于调控多能性、促进端粒延长以及抵抗细胞衰老至关重要。它在小鼠二细胞胚胎的合子基因组激活期间表达，并通过稳定微卫星重复DNA的B-DNA构象来保护胚胎免受DNA损伤。Zscan4能够结合CA/TG微卫星重复序列中的TGCACAC基序，调节端粒延长以维持基因组稳定性，并在未分化的ES细胞中激活自发的端粒姐妹染色单体交换（T-SCE）。在发育过程中或在小鼠胚胎干细胞（mESC）中，Zscan4功能障碍会导致着床失败、端粒维持缺陷以及染色体重排。（参考文献：Srinivasan, R., et al., (2020), Sci. Adv., 6, eaaz9115）
Immunogen
KLH偶联的线性肽，对应于来自小鼠Zscan4蛋白C末端一半的16个氨基酸。该免疫原序列在Zscan4家族蛋白（Zscan4b、Zscan4C、Zscan4d和Zscan4f）之间是保守的。
Application
抗Zscan4抗体（货号07-579）是一种兔多克隆抗体，可检测Zscan4，经过测试可用于蛋白质印迹法。
研究子类别
转录因子
转录因子
研究类别
表观遗传学&核功能
表观遗传学&核功能
Physical form
亲和纯化
纯化的兔多克隆抗体，溶于含有0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠的缓冲液中。
Preparation Note
建议储存：+2°C至+8°C。
Analysis Note
对照
胚胎干细胞裂解物
胚胎干细胞裂解物
通过蛋白质印迹在小鼠胚胎干细胞（mESC）裂解液中进行了评估。 蛋白印迹分析：该抗体稀释1:2,000时可在小鼠胚胎干细胞（mESC）裂解液中检测到Zscan4。
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Lei Yang et al.
EMBO reports, 21(9), e50054-e50054 (2020-07-28)
Cloned animals generated by somatic cell nuclear transfer (SCNT) have been reported for many years; however, SCNT is extremely inefficient, and zygotic genome activation (ZGA) is required for SCNT-mediated somatic cell reprogramming. To identify candidate factors that facilitate ZGA in
Diego Rodriguez-Terrones et al.
Nature genetics, 50(1), 106-119 (2017-12-20)
Unlike pluripotent cells, which generate only embryonic tissues, totipotent cells can generate a full organism, including extra-embryonic tissues. A rare population of cells resembling 2-cell-stage embryos arises in pluripotent embryonic stem (ES) cell cultures. These 2-cell-like cells display molecular features
Ane Iturbide et al.
Nature structural & molecular biology, 28(6), 521-532 (2021-05-29)
Totipotent cells hold enormous potential for regenerative medicine. Thus, the development of cellular models recapitulating totipotent-like features is of paramount importance. Cells resembling the totipotent cells of early embryos arise spontaneously in mouse embryonic stem (ES) cell cultures. Such '2-cell-like-cells'
Diego Rodriguez-Terrones et al.
EMBO reports, 21(1), e48354-e48354 (2019-12-19)
Pluripotent stem cells are thought of as a surrogate of early developmental stages that sustain the capacity to generate all cell types in the body, thereby constituting an invaluable tool to address the mechanisms underlying cellular plasticity. In the mouse
Yuqing Zhu et al.
Stem cell reports, 16(11), 2659-2673 (2021-10-09)
Mouse embryonic stem cells (ESCs) show cell-to-cell heterogeneity. A small number of two-cell-like cells (2CLCs) marked by endogenous retrovirus activation emerge spontaneously. The 2CLCs are unstable and they are prone to transiting back to the pluripotent state without extrinsic stimulus.
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