选择尺寸
关于此项目
western blot
western blot: suitable
产品名称
抗硝基酪氨酸抗体, Chemicon®, from rabbit
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NOS1(4842)
Analysis Note
小鼠大脑(酵母聚糖)的硝基酪氨酸(AB5411)染色,组织用pH 6.0的柠檬酸缓冲液预处理。 使用带有 HRP-DAB 的 IHC-Select Detection 将多克隆抗体稀释至 1:500。免疫反应在海马体中可见。
硝基酪氨酸单克隆的最佳染色: 用酵母聚糖治疗小鼠大脑。
阳性对照:酪氨酸残基可以使用过氧亚硝酸盐(目录号20-107)直接在组织切片上原位硝化,以产生阳性对照。组织切片脱蜡后,在载玻片上滴入pH值为7.4的PBS,其中混合10微升过氧亚硝酸盐溶液。注意溶液的pH值保持在7.4。
Application
氧化应激
神经科学
前一批的1:10 - 1:10 00稀释液成功地用于蛋白质印迹,以检测硝化BSA和硝化卵清蛋白。
免疫组织化学:
1:50-1:100石蜡包埋、10%中性缓冲福尔马林固定小鼠肝脏(经酵母聚糖处理的动物)和正常人小脑组织。组织必须用热诱导表位检索(HIER)预处理。AB5411孵育10分钟,使用Chemicon IHCSelect, HRP-DAB试剂盒(目录号DAB150)产生反应。
最佳工作稀释度必须由最终使用者进行确定。
Biochem/physiol Actions
Disclaimer
General description
Immunogen
Other Notes
Physical form
Preparation Note
Legal Information
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
相关内容
"Redox reactions are powerful chemical processes that involve the reduction and oxidation of proteins and metabolites found in living things. The mechanisms that regulate them are key to maintaining homeostasis and the balance between good health and disease pathology. Oxidative stress is the state where the delicate balance of redox biology is upset, and the pathology of oxidative stress are the cellular consequences to such an imbalance."
Immunoperoxidase Secondary Detection System
RABBIT ANTI-NITROTYROSINE
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