产品名称
抗神经丝H抗体, Chemicon®, from chicken
biological source
chicken
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, pig, bovine, feline, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
bovine ... Nefh(528842)
cat ... Nefh(101087272)
human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)
Analysis Note
对照
阳性对照：脑。
阴性对照：肾，肝。
阳性对照：脑。
阴性对照：肾，肝。
Application
抗神经丝H抗体是针对神经丝的抗体，可用于IC、IH、IH（P）& WB。
蛋白质印迹：使用ECL为1：1,000-1：10,000识别200-220 kDa的条带。
免疫组化：在冷冻或石蜡包埋的组织切片上为1：1,000-1：10,000。建议的固定剂为4%多聚甲醛。
免疫细胞化学：在组织培养中的细胞上为1：1,000-1：10,000，细胞在3.7%福尔马林中固定一分钟，在-20°C甲醇中固定一分钟。
最佳工作稀释度必须由最终用户进行确定。
免疫组化：在冷冻或石蜡包埋的组织切片上为1：1,000-1：10,000。建议的固定剂为4%多聚甲醛。
免疫细胞化学：在组织培养中的细胞上为1：1,000-1：10,000，细胞在3.7%福尔马林中固定一分钟，在-20°C甲醇中固定一分钟。
最佳工作稀释度必须由最终用户进行确定。
Biochem/physiol Actions
识别神经丝重（NF-H）。该抗体染色神经元细胞体（尤其是轴突）中清晰定义的细胞质丝。
General description
200kda
Immunogen
纯化的牛NF-H。
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
在含有5mM叠氮化钠的PBS中纯化的鸡IgY。
形式：纯化
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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