Merck
CN
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AB5543

Sigma-Aldrich

抗 MAP2 抗体

Chemicon®, from chicken

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别名:
Anti-MAP-2, Anti-MAP2A, Anti-MAP2B, Anti-MAP2C
eCl@ss:
32160702
NACRES:
NA.41

生物来源

chicken

质量水平

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

polyclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt登记号

运输

dry ice

target post-translational modification

unmodified

Gene Information

rat ... Map2(25595)

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M2320M9942M1406
Anti-MAP2 Antibody Chemicon®, from chicken

Sigma-Aldrich

AB5543

抗 MAP2 抗体

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

ascites fluid

clone

polyclonal

clone

AP-20, monoclonal

clone

HM-2, monoclonal

clone

AP-20, monoclonal

species reactivity

rat

species reactivity

Xenopus, mouse, quail, human, bovine, rat, aquatic salamander

species reactivity

rat, chicken, human, mouse, bovine, quail

species reactivity

Xenopus, mouse, quail, human, bovine, rat, aquatic salamander

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, microarray: suitable, western blot: 1-3 μg/mL using rat brain preparation or rat cerebral cortex extract

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, microarray: suitable, western blot: 1-2 μg/mL using rat brain extract

technique(s)

immunocytochemistry: suitable, western blot: 1:500 using rat brain enriched microtubule protein preparation or rat cerebral cortex extract

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特异性

微管相关蛋白2(MAP2)。 预计该抗体将与全部哺乳动物反应。

免疫原

纯化牛脑MAP2

应用

抗MAP2抗体是用于 IC、IH& WB的针对MAP2的抗体。
研究子类别
神经 & 胶质标志物

神经丝 & 神经元代谢
研究类别
神经科学
蛋白质印迹:使用ECL为1:5,000-1:10,000识别约280 kDa的双峰和约70 kDa的双峰。

在冷冻组织切片上进行免疫组织化学分析:建议的固定剂是4%多聚甲醛(1-10分钟),然后在-20°C甲醇中5分钟。

免疫细胞化学:在组织培养中的细胞上为1:5,000-1:10,000,在10%福尔马林中固定1-10分钟,然后在-20°C甲醇中固定5分钟。

最佳工作稀释度必须由最终用户进行确定。

目标描述

识别约280 kDa的双峰和约70 kDa的双峰

外形

形式:纯化
硫酸铵沉淀
纯化的鸡IgY。 含有5 mM叠氮化钠的PBS液体。

储存及稳定性

自发运之日起在-20°C可保存1年。等分以避免反复冻融。为了最大程度地回收产品,在融化后和取下盖子之前,将原始样品瓶进行离心。

分析说明

对照
阳性=大脑皮层。阴性=肝脏或肾脏。

其他说明

浓度:请参考批次特异性浓缩物的分析证书。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

储存分类代码

10 - Combustible liquids

WGK

WGK 2


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