AB6017
抗F-肌动蛋白加帽蛋白亚基β抗体
from rabbit
别名:
capping protein (actin filament) muscle Z-line, beta, F-actin capping protein beta subunit
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunocytochemistry
western blot
western blot
Species reactivity:
mouse, rat, human
Citations:
14
Technique(s):
immunocytochemistry: suitable
western blot: suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗F-肌动蛋白加帽蛋白亚基β抗体, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
species reactivity (predicted by homology)
canine (based on 100% sequence homology), primate (based on 100% sequence homology), bovine (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
bovine ... Capzb(338052)
dog ... Capzb(478209)
human ... CAPZB(832)
mouse ... Capzb(12345)
rat ... Capzb(298584)
Analysis Note
对照
HeLa细胞裂解液
HeLa细胞裂解液
通过 HeLa 细胞裂解液中的蛋白质印迹进行了评估。
蛋白质印迹分析：0.1 µg/mL该抗体在10 µg HeLa细胞裂解物中检测到F-肌动蛋白加帽蛋白亚基β。
蛋白质印迹分析：0.1 µg/mL该抗体在10 µg HeLa细胞裂解物中检测到F-肌动蛋白加帽蛋白亚基β。
Application
使用经验证可用于WB& IC的抗F-肌动蛋白加帽蛋白亚基β抗体检测F-肌动蛋白加帽蛋白亚基β。
免疫细胞化学分析： 已显示抗体的1：500稀释液可检测NIH/3T3和A431细胞中的F-肌动蛋白加帽蛋白亚基β。
研究子类别
细胞骨架
细胞骨架
研究类别
细胞结构
细胞结构
Biochem/physiol Actions
该抗体可识别亚型2 C端的F-肌动蛋白加帽蛋白亚基β。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
F-肌动蛋白加帽蛋白亚基β（CapZ beta）属于F-肌动蛋白加帽蛋白家族，是特征异二聚体，由一个α亚基（31-36 kDa）和一个β亚基（28-32 kDa）组成，加帽所有真核生物内肌动蛋白丝的带刺末端。它们结合肌动蛋白丝的能力的方式独立于Ca2++，并且需要两个亚基的C末端才能实现最佳结合。F-肌动蛋白加帽蛋白亚基β包含在横纹肌的Z盘中，在其中它起到抑制肌动蛋白聚合和解聚的作用。在脊椎动物中，每个F-肌动蛋白加帽蛋白亚基β’亚基有三种亚型。虽然关于β3亚基几乎一无所知，但β1和β2亚型各自具有不同的作用。亚型β2主要在非肌肉细胞中表达，并在闰盘和细胞周围发现，但在Z盘上未观察到。亚型β1在横纹肌中表达更多，并位于Z盘上。它还包含加帽肌动蛋白所需的COOH末端延伸。
观察到的分子量~ 34 kDa。 选择性剪接产生3种亚型：31 kDa的亚型1，31 kDa的亚型2和亚型3（序列不可用）。
Immunogen
对应于亚型2 C端的人F-肌动蛋白加帽蛋白亚基β的KLH偶联线性肽。
表位：亚型2的C末端
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
在含 0.1 M Tris-甘氨酸（pH 7.4），150 mM NaCl 和 0.05％ 叠氮化钠的缓冲液中的纯化的兔多克隆抗体。
形式：纯化
纯化的蛋白 A
Preparation Note
自收到之日起，在 2-8°C 条件下可稳定保存1年
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Shamim A Sinnar et al.
Molecular biology of the cell, 25(14), 2152-2160 (2014-05-16)
Capping protein (CP) binds to barbed ends of growing actin filaments and inhibits elongation. CP is essential for actin-based motility in cell-free systems and in Dictyostelium. Even though CP is believed to be critical for creating the lamellipodial actin structure
Haibo Du et al.
Frontiers in cell and developmental biology, 9, 765559-765559 (2021-11-09)
Stereocilia are actin-based cell protrusions on the apical surface of inner ear hair cells, playing a pivotal role in hearing and balancing sensation. The development and maintenance of stereocilia is tightly regulated and deficits in this process usually lead to
Ying-Hsi Lin et al.
Journal of muscle research and cell motility, 36(4-5), 329-337 (2015-10-03)
The heart is exquisitely sensitive to mechanical stimuli and adapts to increased demands for work by enlarging the cardiomyocytes. In order to determine links between mechano-transduction mechanisms and hypertrophy, neonatal rat ventricular myocytes (NRVM) were subjected to physiologic strain for
Keji Yan et al.
Frontiers in molecular neuroscience, 15, 829204-829204 (2022-03-05)
Hair cells are mechanosensitive cells in the inner ear, characterized by dozens to hundreds of actin-based stereocilia and one tubulin-based kinocilium on the apical surface of each cell. Two types of hair cells, namely cochlear hair cells and vestibular hair
Erin F Spence et al.
Nature communications, 10(1), 386-386 (2019-01-25)
Excitatory synapse formation during development involves the complex orchestration of both structural and functional alterations at the postsynapse. However, the molecular mechanisms that underlie excitatory synaptogenesis are only partially resolved, in part because the internal machinery of developing synapses is
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