AB755P
Anti-Collagen Type I (COL1A1) Antibody
CHEMICON®, rabbit polyclonal
别名:
Anti-CAFYD, Anti-EDSC, Anti-OI1, Anti-OI2, Anti-OI3, Anti-OI4
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关于此项目
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
rat
Application:
ELISA, ICC, IHC, RIA
Citations:
48
产品名称
抗大鼠胶原I型抗体, Chemicon®, from rabbit
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), radioimmunoassay: suitable
isotype
IgG
suitability
not suitable for Western blot
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... COL1A1(1277)
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General description
从大鼠皮肤中提取纯化的I型胶原。 该抗体与人、小鼠、鸡ⅰ型胶原蛋白、大鼠II、III、V型胶原蛋白和大鼠弹力蛋白的反应性低于0.1%。
Immunogen
从大鼠皮肤中提取纯化的I型胶原。
Application
ELISA：
一个先前批次的1:200稀释液被用于ELISA。
RIA：
先前批次的抗体用于RIA。
免疫细胞化学：
使用先前批次的1:40-1:80稀释液对冷冻小鼠皮肤和肝脏组织进行免疫荧光染色。
也可用于丙酮固定细胞。
免疫组织化学：
1:40-1:80稀释液用于冰冻大鼠皮肤和肝脏组织的免疫荧光染色。使用ABC检测系统时，该抗体的1:500稀释液在石蜡包埋的大鼠组织（皮肤、肝脏）上也有反应。
不建议用于蛋白质印迹。
最佳工作稀释度必须由最终用户确定。
一个先前批次的1:200稀释液被用于ELISA。
RIA：
先前批次的抗体用于RIA。
免疫细胞化学：
使用先前批次的1:40-1:80稀释液对冷冻小鼠皮肤和肝脏组织进行免疫荧光染色。
也可用于丙酮固定细胞。
免疫组织化学：
1:40-1:80稀释液用于冰冻大鼠皮肤和肝脏组织的免疫荧光染色。使用ABC检测系统时，该抗体的1:500稀释液在石蜡包埋的大鼠组织（皮肤、肝脏）上也有反应。
不建议用于蛋白质印迹。
最佳工作稀释度必须由最终用户确定。
抗大鼠I型胶原抗体是一种用于ELISA、IC、IH(P)、RIA的I型胶原抗体。
Biochem/physiol Actions
识别大鼠I型胶原。
该抗体与人、小鼠、鸡ⅰ型胶原蛋白、大鼠II、III、V型胶原蛋白和大鼠弹力蛋白的反应性低于0.1%。
Physical form
纯化抗体溶于缓冲液中，内含0.1M柠檬酸盐、0.1M磷酸钾盐(pH 7.2-7.4)和10 µg/ml抗生素和抗真菌剂
Analysis Note
免疫组化（石蜡）：
大鼠皮肤中的I型胶原蛋白（目录号AB755P）染色模式/形态。组织用枸橼酸盐（pH 6.0）抗原修复法预处理。使用带有HRP-DAB的IHC-Select试剂，将该批次抗体稀释成1:1000。 石蜡切片纤维染色可见免疫反应性。注意，染色模式与预期一致。
免疫组化石蜡染色与表位检索：大鼠皮肤
大鼠皮肤中的I型胶原蛋白（目录号AB755P）染色模式/形态。组织用枸橼酸盐（pH 6.0）抗原修复法预处理。使用带有HRP-DAB的IHC-Select试剂，将该批次抗体稀释成1:1000。 石蜡切片纤维染色可见免疫反应性。注意，染色模式与预期一致。
免疫组化石蜡染色与表位检索：大鼠皮肤
对照
大鼠肝星状细胞裂解液
大鼠肝星状细胞裂解液
Other Notes
浓度：请参考批次特异性浓缩物的检验报告。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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