产品名称
抗雷诺丁受体3抗体, serum, Chemicon®
biological source
rabbit
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse, human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... RYR3(6263)
Preparation Note
自收到之日起，在 -20°C 以未稀释的等分试样可保存 6 个月。应避免反复冻/融循环。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Application
该抗雷诺丁受体3抗体经过验证可用在IC、IH、WB 中检测雷诺丁受体3。
研究子类别
离子通道&转运蛋白
信号传导神经科学
离子通道&转运蛋白
信号传导神经科学
研究类别
神经科学
神经科学
蛋白质印迹：使用ECL为1：1,000。该抗体与500 kDa雷诺丁受体3蛋白反应。建议的封闭缓冲液为10%正常山羊血清（或与你的二抗相同的宿主），1%BSA的含0.05%吐温20的0.1 M PBS。建议的稀释缓冲液为1%正常山羊血清（或与你的二抗相同的宿主），1%BSA的含0.05%吐温20的0.1 M PBS。优选的凝胶百分比是4-12%梯度凝胶。
免疫细胞化学：1：1,000
免疫组化：使用荧光标记的二抗在2-8°C下以1：1,000过夜。建议的固定剂是0.1 M PBS中的4%多聚甲醛（一小时）。建议的透化方法是0.05%Triton X-100稀释缓冲液。建议的封闭缓冲液为10%正常山羊血清（或与你的二抗相同的宿主）和1%BSA的0.1 M PBS。建议稀释缓冲液为含1%正常山羊血清（或与二抗相同的宿主）和1%BSA的0.1 M PBS。最佳工作稀释度必须由最终用户确定。
免疫细胞化学：1：1,000
免疫组化：使用荧光标记的二抗在2-8°C下以1：1,000过夜。建议的固定剂是0.1 M PBS中的4%多聚甲醛（一小时）。建议的透化方法是0.05%Triton X-100稀释缓冲液。建议的封闭缓冲液为10%正常山羊血清（或与你的二抗相同的宿主）和1%BSA的0.1 M PBS。建议稀释缓冲液为含1%正常山羊血清（或与二抗相同的宿主）和1%BSA的0.1 M PBS。最佳工作稀释度必须由最终用户确定。
Biochem/physiol Actions
雷诺丁受体3。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Immunogen
来自人雷诺丁受体3变体TM区的合成肽。
Analysis Note
对照
蛋白质印迹=成年小鼠脑（成年小鼠骨骼肌为阴性）。
IHC=成年小鼠小脑浦肯野细胞（成年小鼠小脑颗粒细胞为阴性）。
蛋白质印迹=成年小鼠脑（成年小鼠骨骼肌为阴性）。
IHC=成年小鼠小脑浦肯野细胞（成年小鼠小脑颗粒细胞为阴性）。
Physical form
兔血清。 液体。
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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