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Merck
CN

ABC118

抗磷酸Beclin抗体（Thr119）

from rabbit, purified by affinity chromatography

别名:

Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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产品名称

抗磷酸Beclin抗体（Thr119）, from rabbit, purified by affinity chromatography

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), mouse (based on 100% sequence homology), porcine (based on 100% sequence homology)

technique(s)

inhibition assay: suitable (peptide)
western blot: suitable

NCBI accession no.

NP_003757

UniProt accession no.

Q14457

shipped in

wet ice

target post-translational modification

phosphorylation (pThr119)

Quality Level

100

Gene Information

human ... BECN1(8678)

相关类别

Analysis Note

对照
人前列腺肿瘤组织裂解物。
通过蛋白质印迹对人前列腺肿瘤组织裂解物进行了评估。

蛋白质印迹分析：5 µg/mL该抗体检测到10 µg 人前列腺肿瘤组织裂解物。

Application

使用经验证可用于蛋白质印迹&肽抑制试验的兔多克隆抗体（抗磷酸化Beclin抗体（Thr119））检测Beclin。
研究子类别
凋亡-附加
研究类别
细胞凋亡 & 癌症
蛋白质印迹/肽抑制分析：5 µg/mL代表性批次在LNCap细胞裂解物中检测到磷酸化Beclin（Thr119）。在添加磷酸肽的情况下观察到肽抑制作用，但在添加非磷酸肽的情况下未观察到肽抑制作用。

Biochem/physiol Actions

该抗体可识别Thr119磷酸化的Beclin的BH3结构域。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

Beclin-1（BECN1）基因编码一个 60 kDa 的卷曲螺旋蛋白，该蛋白与原型凋亡抑制因子 Bcl-2相互作用。Beclin-1 促进 agp6/vps30 靶向缺失的自噬缺陷型酵母菌发生自噬，也促进人 MCF7 乳腺癌细胞发生自噬。Beclin-1 在 MCF7 细胞中的自噬促进活性与 MCF7 细胞增殖的抑制、体外克隆性和肿瘤发生有关，并且人们发现其可减轻帕金森′氏症和路易体病的 α-突触核蛋白模型的神经退行性病变。内源性Beclin-1蛋白在人乳腺上皮癌细胞系和组织中通常表达水平较低，但在正常乳腺上皮细胞中普遍表达水平较高。研究还表明，Beclin 1含有富含亮氨酸的核输出信号基序，从而增加了其自噬和/或肿瘤抑制功能可能需要受调节的、依赖CRM1的核质转运的可能性。
观察值〜50 kDa。在某些细胞裂解物中可能会观察到未表征的条带。

Immunogen

KLH偶联的线性肽，对应于在Thr119磷酸化的人Beclin的BH3结构域。
表位：BH3结构域

Other Notes

浓度：请参考批次特异性浓缩物的分析证书。

Physical form

亲和纯化
纯化的兔多克隆血清，溶于含有0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠的缓冲液中。

Preparation Note

自收到之日起，在 2-8°C 条件下可稳定保存1年

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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