ABD69
抗巢蛋白，人抗体
serum, from rabbit
别名:
Nestin
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunocytochemistry
immunohistochemistry
western blot
immunohistochemistry
western blot
Species reactivity:
human
Citations:
74
Technique(s):
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
immunohistochemistry: suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗巢蛋白，人抗体, serum, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
should not react with
rat, mouse
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NES(10763)
Analysis Note
对照
U251胶质母细胞瘤细胞。
U251胶质母细胞瘤细胞。
通过免疫细胞化学在人U251胶质母细胞瘤细胞中进行评价。 免疫细胞化学分析：该抗体的1：500稀释液在人U251胶质母细胞瘤细胞检测到巢蛋白。
Application
使用经验证可用于蛋白质印迹法，ICC &IHC的抗巢蛋白人抗体检测人巢蛋白。
通过免疫细胞化学在人WI38细胞中进行评估。
免疫细胞化学分析：该抗体的1：500稀释液在人WI38细胞中检测到巢蛋白。
通过免疫组化在人胶质母细胞瘤组织中进行评价。
免疫细胞化学分析：该抗体的1：2,000稀释液在人胶质母细胞瘤组织中检测到巢蛋白。
通过蛋白质印迹在人U251胶质母细胞瘤细胞中进行评价。蛋白质印迹分析：已显示该抗体的1：25,000稀释液可在人U251胶质母细胞瘤细胞中检测到巢蛋白。
免疫细胞化学分析：该抗体的1：500稀释液在人WI38细胞中检测到巢蛋白。
通过免疫组化在人胶质母细胞瘤组织中进行评价。
免疫细胞化学分析：该抗体的1：2,000稀释液在人胶质母细胞瘤组织中检测到巢蛋白。
通过蛋白质印迹在人U251胶质母细胞瘤细胞中进行评价。蛋白质印迹分析：已显示该抗体的1：25,000稀释液可在人U251胶质母细胞瘤细胞中检测到巢蛋白。
Biochem/physiol Actions
该抗体可识别人类巢蛋白。抗体不与大鼠和小鼠巢蛋白反应。
Disclaimer
仅供研究使用在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。
General description
巢蛋白，一种在发育过程中以及在肌腱和神经肌肉接头中表达的大型中间丝蛋白（VI型）。巢蛋白表达受到限制，通常在E18时消失。巢蛋白被认为是一种合理的神经元标记物。然而，最近的研究发现巢蛋白也在其他类型的细胞如内皮细胞中表达{Folia Biologica, 44(5):155, 1998}。巢蛋白可识别最原始的神经上皮细胞，但也可以识别许多其他胚胎组织，因此它对CNS并不特异。巢蛋白的表达在几乎所有GBM（成胶质母细胞瘤）和许多黑色素瘤（原发性和转移性）中均可见，但在所有转移性癌中均未发现。
抗巢蛋白抗体分子量为220kDa，抗体在某些细胞和/或处理中可见到260kDa的条带
Immunogen
GST 标记的重组蛋白，对应于人巢蛋白。
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存储类别
10 - Combustible liquids
wgk
WGK 1
Sutapa Ray et al.
Molecular carcinogenesis, 57(4), 536-548 (2017-12-28)
Medulloblastoma (MB) is a malignant pediatric brain tumor with poor prognosis. Signal transducers and activators of transcription-3 (STAT3) is constitutively activated in MB where it functions as an oncoprotein, mediating cancer progression and metastasis. Here, we have delineated the functional
Smita Jagtap et al.
Human molecular genetics, 28(21), 3625-3636 (2019-09-14)
The X-linked neurodevelopmental diseases CDKL5 deficiency disorder (CDD) and Rett syndrome (RTT) are associated with intellectual disability, infantile spasms and seizures. Although mitochondrial dysfunction has been suggested in RTT, less is understood about mitochondrial function in CDD. A comparison of
Intraspinal transplantation of mouse and human neural precursor cells.
Weinger, JG; Chen, L; Coleman, R; Leang, R; Plaisted, WC; Loring, JF; Lane, TE
Current Protocols in Stem Cell Biology null
Paul M George et al.
Biomaterials, 142, 31-40 (2017-07-19)
Exogenous human neural progenitor cells (hNPCs) are promising stroke therapeutics, but optimal delivery conditions and exact recovery mechanisms remain elusive. To further elucidate repair processes and improve stroke outcomes, we developed an electrically conductive, polymer scaffold for hNPC delivery. Electrical
Zong-Yu Xiao et al.
Turkish neurosurgery, 28(6), 910-914 (2018-01-26)
To investigate the expression of cancer stem cell markers in meningiomas. CD133, Nestin and Sox2 expression levels in 35 paraffin-embedded meningioma tissue samples were assessed using immunohistochemistry. In this study, five cases were atypical (WHO Grade II), two were anaplastic
实验方案
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
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