ABE1851
抗-MLL-3
from rabbit
别名:
HALR, Histone-lysine N-methyltransferase 2C, EC: 2.1.1.43, Lysine-N-methyltransferase 2C, Myeloid-lineage leukemia protein 3 homolog
登录 查看组织和合同定价。
选择尺寸
关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ChIP
immunohistochemistry
western blot
immunohistochemistry
western blot
Species reactivity:
rat, human, mouse
Citations:
6
Technique(s):
ChIP: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Uniprot accession no.:
产品名称
抗-MLL-3, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, human, mouse
packaging
antibody small pack of 25 μg
technique(s)
ChIP: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Quality Level
Gene Information
human ... KMT2C(58508)
mouse ... Kmt2C(231051)
Analysis Note
通过蛋白质印迹在U2OS核提取物中进行了评估。
蛋白质印迹分析：1 µg/mL该抗体在 10 µg U2OS细胞核提取物中检测到MLL-3。
蛋白质印迹分析：1 µg/mL该抗体在 10 µg U2OS细胞核提取物中检测到MLL-3。
Application
免疫组化分析：代表性批次的1：1,000稀释液在小鼠睾丸，大鼠子宫和小鼠肾脏组织中检测到MLL-3。
染色质免疫沉淀分析：代表性批次在MEF细胞中检测到MLL-3（Lee, J., et. al. (2009) Proc Natl Acad Sci USA.106(21):8513-8）。
蛋白质印迹分析：代表性批次在蛋白质印迹应用中检测到MLL-3（Lee, J., et. al. (2009) Proc Natl Acad Sci USA.106(21):8513-8); Bres, V., et. al. (2009).Mol Cell.36(1):75-87)。
免疫组化分析：代表性批次在小鼠尿路上皮肿瘤中检测到MLL-3（Lee, J., et. al. (2009) Proc Natl Acad Sci USA.106（21）：8513-8）。
染色质免疫沉淀分析：代表性批次在MEF细胞中检测到MLL-3（Lee, J., et. al. (2009) Proc Natl Acad Sci USA.106(21):8513-8）。
蛋白质印迹分析：代表性批次在蛋白质印迹应用中检测到MLL-3（Lee, J., et. al. (2009) Proc Natl Acad Sci USA.106(21):8513-8); Bres, V., et. al. (2009).Mol Cell.36(1):75-87)。
免疫组化分析：代表性批次在小鼠尿路上皮肿瘤中检测到MLL-3（Lee, J., et. al. (2009) Proc Natl Acad Sci USA.106（21）：8513-8）。
抗MLL3，目录号ABE1851是一种高度特异性的兔多克隆抗体，靶向组蛋白-赖氨酸N-甲基转移酶2C，并已在染色质免疫沉淀（ChIP），免疫组化（石蜡）和蛋白质印迹中进行了测试。
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
该多克隆抗体可检测人，小鼠和大鼠细胞中的MLL-3蛋白。它靶向N末端一半内部区域的18个氨基酸内表位。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
组蛋白赖氨酸N-甲基转移酶2D（EC 2.1.1.43；UniProt O14686；也称为MLL4，ALL1相关蛋白，赖氨酸N-甲基转移酶2D）由人的KMT2D（也称为MLL4，ALR，KABUK1，MLL2）基因（基因ID：8085）所编码。MLL4使组蛋白H3的Lys-4甲基化，形成H3K4单甲基化和二甲基化（H3K4me1/2），它代表转录增强子的特异性标签。MLL4也是MLL3/4蛋白复合物的一个组分，在细胞分化和动物发育过程中发挥重要作用。MLL4定位于细胞核，并在大多数胚胎和成年组织中表达。MLL4的缺陷（在文献中通常称为MLL2和KMT2D）与歌舞伎综合征和先天性心脏病有关。MLL4/MLL2/KMT2D基因的失活性突变与许多类型的癌症有关，包括髓母细胞瘤，非霍奇金淋巴瘤，弥漫性大B细胞淋巴瘤，前列腺癌，乳腺癌，肝细胞癌，卵巢癌，肺癌，膀胱癌，头&颈癌和胰腺癌。（参考文献：Lee, J.E., et al. (2013).Elife.2:e01503; Lawrence, M.S., et al., (2014).Nature.505（7484）：495-501）。
观察分子量为〜540 kDa；计算分子量为540.19 kDa。在某些裂解物中可以观察到未鉴定的条带。
Immunogen
线性肽对应人MLL-3 N末端区域的18个氨基酸。
表位：未知
Other Notes
浓度：请参考特定批次的数据表。
Physical form
亲和纯化
形式：纯化
纯化的兔多克隆抗体，溶于含有0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠的缓冲液中。
Preparation Note
自收到之日起，在 2-8°C 条件下可稳定保存1年
未找到合适的产品？
试试我们的产品选型工具.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Takumi Nakamura et al.
Molecular psychiatry (2024-03-26)
Recent studies have consistently demonstrated that the regulation of chromatin and gene transcription plays a pivotal role in the pathogenesis of neurodevelopmental disorders. Among many genes involved in these pathways, KMT2C, encoding one of the six known histone H3 lysine
Ran Chen et al.
Cell reports, 34(7), 108751-108751 (2021-02-18)
The myeloid tumor suppressor KMT2C is recurrently deleted in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML), particularly therapy-related MDS/AML (t-MDS/t-AML), as part of larger chromosome 7 deletions. Here, we show that KMT2C deletions convey a selective advantage to hematopoietic
Jun-Yi Zheng et al.
Cell death & disease, 12(4), 364-364 (2021-04-08)
MLL3 is a histone H3K4 methyltransferase that is frequently mutated in cancer, but the underlying molecular mechanisms remain elusive. Here, we found that MLL3 depletion by CRISPR/sgRNA significantly enhanced cell migration, but did not elevate the proliferation rate of cancer
Yingxue Wang et al.
Molecular therapy. Nucleic acids, 24, 610-621 (2021-04-27)
Papillary thyroid cancer (PTC) is the most common type of thyroid cancer, and angiogenesis plays critical roles in its recurrence and metastasis. In this study, we investigated the effects of hypoxia-induced exosomal microRNA-181 (miR-181a) from PTC on tumor growth and
Masaki Suzuki et al.
Translational lung cancer research, 12(8), 1738-1751 (2023-09-11)
High-grade fetal adenocarcinoma of the lung (H-FLAC) is a rare variant of pulmonary adenocarcinoma. Our previous study showed a high frequency of KMT2C mutations in lung cancers with an H-FLAC component, showing that KMT2C dysfunction may be associated with the
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持