ABN101
抗-GAD65抗体
rabbit polyclonal
别名:
Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform
产品名称
抗-GAD65抗体, from rabbit, purified by affinity chromatography
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human, rat, pig
species reactivity (predicted by homology)
canine (based on 100% sequence homology), porcine (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GAD2(2572)
Analysis Note
对照
大鼠脑膜组织裂解物
大鼠脑膜组织裂解物
通过蛋白质印迹在大鼠脑膜组织裂解物中进行了评估。
蛋白质印迹分析：1 µg/mL该抗体在10 µg大鼠脑膜组织裂解物中检测到GAD65。
蛋白质印迹分析：1 µg/mL该抗体在10 µg大鼠脑膜组织裂解物中检测到GAD65。
Application
抗GAD抗体是可用于蛋白质印迹的抗GAD的抗体。有关该抗GAD抗体的更多信息，请参见下文。
研究子类别
发育神经科学
发育神经科学
研究类别
神经科学
神经科学
蛋白质印迹分析：1 µg/mL代表性批次在10 µg的大鼠脑膜组织裂解物中检测到GAD65。 在没有吐温的情况下进行蛋白质印迹。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
GAD65是II类脱羧酶，可借助辅因子5′-磷酸盐催化谷氨酸生物合成GABA。GAD65位于突触前的GABA能端，据报告可提供GABA的局部囊泡池，从而促进瞬时抑制性神经传递，尽管GAD65也可能在更持久的强直抑制信号中起作用。其作为α和β亚基的二聚体存在，并受选择性剪接调节，可产生GAD67亚型。GAD65还经历翻译后修饰，例如N末端半胱氨酸残基的棕榈酰化和多个α-亚基丝氨酸残基的磷酸化，从而实现膜锚定和其他囊泡活性。GAD65与抑制性神经传递失败导致的神经系统疾病有关，例如亨廷顿氏′病和癫痫。除了其CNS的存在外，GAD65还在胰腺β细胞中产生GABA，该区域的功能障碍可能与胰岛素依赖型糖尿病的发病机理有关。
观察值〜59/63 kDa。计算的分子量为65 kDa，但是GAD65在蛋白质印迹中显示为〜59/63双峰。（Christgau, S., et al. (1991). Journal of Biological Chemistry.266（31）：21257-21264.） 在某些裂解物中，未表征的条带出现在〜38 kDa处。
Immunogen
对应于人GAD65的KLH偶联线性肽。
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
亲和纯化
在含 0.1 M Tris-甘氨酸（pH 7.4），150 mM NaCl 和 0.05％ 叠氮化钠的缓冲液中的纯化的兔多克隆抗体。
Preparation Note
自收到之日起，在 2-8°C 条件下可稳定保存1年
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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