ABN37
抗-Homer1抗体
from rabbit, purified by affinity chromatography
别名:
Homer protein homolog 1, Homer-1, Homer1
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunohistochemistry
western blot
western blot
Species reactivity:
human, mouse
Citations:
18
Technique(s):
immunohistochemistry: suitable (paraffin)
western blot: suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗-Homer1抗体, from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
species reactivity (predicted by homology)
horse (based on 100% sequence homology), monkey (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... HOMER1(9456)
Biochem/physiol Actions
该兔多克隆抗体靶向人Homer1亚型1b和小鼠亚型Vesl-1L N端一半内的内部序列。免疫原序列出现在人亚型1h和小鼠亚型1b，1d，Vesl-1M和Vesl-1S的C末端附近，但在人亚型1e中不存在。在大鼠亚型1c（Vesl-1L）的N末端一半和大鼠亚型1a（Vesl）和1b的C末端附近也发现了高度同源的序列（〜83%）。
由于序列同源性高，预期具有广泛的物种反应性。
Analysis Note
通过蛋白质印迹在小鼠脑组织裂解液中进行了评估。
蛋白质印迹分析：2 µg/mL的该抗体可在10 µg小鼠脑组织裂解物中检测到Homer1。
蛋白质印迹分析：2 µg/mL的该抗体可在10 µg小鼠脑组织裂解物中检测到Homer1。
Application
免疫组化分析：代表性批次的1：500稀释液在人和小鼠大脑皮层组织切片中检测到Homer1。
免疫组化分析：代表性批次在接受AP-12腹膜内注射的小鼠的浮动脑切片中检测到放射层和始层Homer1免疫反应性显著增加（Jansone, B., et al. (2015).PLoS One.10(6):e0127686）。
免疫组化分析：代表性批次在接受AP-12腹膜内注射的小鼠的浮动脑切片中检测到放射层和始层Homer1免疫反应性显著增加（Jansone, B., et al. (2015).PLoS One.10(6):e0127686）。
抗Homer1，目录号ABN37是一种高度特异性的兔多克隆抗体，靶向Homer蛋白同源物1，并已在免疫组化和蛋白质印迹中进行了测试。
General description
Homer蛋白同源物1（UniProt Q86YM7；也称为Homer-1，Homer1）由人的HOMER1（也称为SYN47）基因（基因 ID 9456）编码。Homer-1，-2和-3构成突触后支架蛋白家族，在钙信号传导中起着核心作用。Homer成员共享一个保守的N末端Ena/VASP同源物1（EVH1）域（Homer-1的a.a.1-110），通过与在各种其他支架和信号转导分子（包括代谢型谷氨酸受体（mGluRs）和1，4，5-三磷酸受体（IP3Rs））中发现的PPXXFR富含脯氨酸的共有序列相互作用来介导信号复合物组装。除了通过其EVH1结构域介导I组mGluR信号传导外，Homer-1剪接的变体Vesl-1L，1b和1d，而非Vesl-1M或Vesl-1S，还包含C末端卷曲螺旋（a.a.181-352）域，其可介导自身多聚化。Homer-1变体与许多神经系统疾病的病因有关，例如慢性疼痛，精神发育迟缓综合征，阿尔茨海默′病（AD），帕金森′病（PD），精神分裂症，药物性成瘾和创伤性脑损伤（TBI）。据报告，Homer1b/c-基因沉默通过阻止mGluR5介导的mTOR途径激活，可以防止化学诱导的小鼠惊厥发作。
观察值〜47 kDa。计算值40.28/19.86 kDa（人亚型1b/1h），40.22/42.08/41.41/22.78/20.91 kDa（小鼠亚型1b/1d/Vesl-1L/Vesl-1M/Vesl-1S）。在某些裂解物中可以观察到未鉴定的条带。
Immunogen
对应于人Homer1亚型1b N-末端一半内和亚型1h C-末端附近的内部序列的KLH偶联线性肽。
Other Notes
浓度：请参考特定批次的数据表。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Yuvraj Joshi et al.
The Journal of physiology, 599(24), 5397-5416 (2021-11-17)
DFNA25 is an autosomal-dominant and progressive form of human deafness caused by mutations in the SLC17A8 gene, which encodes the vesicular glutamate transporter type 3 (VGLUT3). To resolve the mechanisms underlying DFNA25, we studied phenotypes of mice harbouring the p.A221V
A Novel 1,4-Dihydropyridine Derivative Improves Spatial Learning and Memory and Modifies Brain Protein Expression in Wild Type and Transgenic APPSweDI Mice.
Jansone, B; Kadish, I; van Groen, T; Beitnere, U; Moore, DR; Plotniece, A; Pajuste, K; Klusa, V
Testing null
Jie Wang et al.
Cell, 184(24), 5869-5885 (2021-11-11)
RTN4-binding proteins were widely studied as "NoGo" receptors, but their physiological interactors and roles remain elusive. Similarly, BAI adhesion-GPCRs were associated with numerous activities, but their ligands and functions remain unclear. Using unbiased approaches, we observed an unexpected convergence: RTN4
Baiba Jansone et al.
Pharmacological research, 113(Pt B), 781-787 (2016-06-28)
The prevalence of Alzheimer's disease (AD) is higher in females than in males, and causes more severe cognitive, memory and behavioral impairments. Previously, in male transgenic (Tg) APPSweDI mice, we reported that the novel lipophilic 1,4-dihydropyridine (DHP) derivative AP-12 crossed
Shuai Wang et al.
Nature, 626(7997), 128-135 (2024-01-18)
The assembly and specification of synapses in the brain is incompletely understood1-3. Latrophilin-3 (encoded by Adgrl3, also known as Lphn3)-a postsynaptic adhesion G-protein-coupled receptor-mediates synapse formation in the hippocampus4 but the mechanisms involved remain unclear. Here we show in mice
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