产品名称
抗神经丝H(200 kDa)抗体, from rabbit, purified by affinity chromatography
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human, rat, bovine
technique(s)
immunohistochemistry: suitable (paraffin), western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
bovine ... Nefh(528842)
human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)
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General description
神经丝是中间丝的一种,是支持轴突细胞质的细胞骨架的主要成分。它们是轴突中最丰富的纤维成分,在平均值上的频率是轴突微管的3-10倍。神经丝(直径10nm)由三个相互缠绕的原纤维构建而成,原纤维本身由单体蛋白质的两个四聚体原丝复合物组成。神经丝三联体蛋白(68/70、160和200 kDa)同时存在于中枢和外周神经系统中,通常是神经元特异性的。68/70 kDa NF-L蛋白可以自组装成丝状结构,但是160 kDa NF-M和200 kDa NF-H蛋白需要68/70 kDa NF-L蛋白的存在才能共同组装。各种神经瘤,神经节神经瘤,神经节神经胶质瘤,神经节神经母细胞瘤和神经母细胞瘤的神经丝染色呈阳性。尽管通常仅限于神经元,但已在副神经节瘤,肾上腺和肾上腺外嗜铬细胞瘤,类癌,皮肤神经内分泌癌和肺燕麦细胞癌中检测到神经丝。有关更多信息,请参见Millipore网站上的神经系统细胞类型特异性标志物图表。
观察值〜200 kDa
Immunogen
GST 标记的重组蛋白,对应于牛神经丝H。
Application
免疫组化分析:代表性批次的1 µg/mL稀释液在正常大鼠小脑组织中检测到神经丝H。
该抗神经丝H(200 kDa)抗体经验证可用于WB,IH(P)中检测神经丝H(200 kDa)。
Analysis Note
对照
牛小脑组织裂解物
牛小脑组织裂解物
通过蛋白质印迹在牛小脑组织裂解物中进行了评估。
蛋白质印迹分析:0.1 µg/mL该抗体在牛小脑组织裂解物中检测到神经丝H。
蛋白质印迹分析:0.1 µg/mL该抗体在牛小脑组织裂解物中检测到神经丝H。
Other Notes
替代品:AB1982
浓度:请参考批次特异性浓缩物的分析证书。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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