ABS233
抗PI3激酶p85抗体，N-SH2域
from rabbit, purified by affinity chromatography
别名:
Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-al
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunoprecipitation (IP)
western blot
western blot
Species reactivity:
human, rat
Citations:
15
Technique(s):
immunoprecipitation (IP): suitable
western blot: suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗PI3激酶p85抗体，N-SH2域, from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, rat
species reactivity (predicted by homology)
mouse (based on 100% sequence homology), monkey (based on 100% sequence homology), bovine (based on 100% sequence homology)
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
bovine ... Pik3R1(282307)
human ... PIK3R1(5295)
mouse ... Pik3R1(18708)
rat ... Pik3R1(25513)
rhesus monkey ... Pik3R1(698996)
Other Notes
浓度：请参考特定批次的数据表。
替代品：06-496
Physical form
亲和纯化
纯化的兔多克隆抗体，溶于含有0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠的缓冲液中。
Preparation Note
自收到之日起，在 2-8°C 条件下可稳定保存1年
Analysis Note
对照
Jurkat 细胞裂解液
Jurkat 细胞裂解液
通过蛋白质印迹对大鼠脑组织裂解液进行了评估。
蛋白质印迹分析：0.5 µg/ml该抗体在10µg大鼠脑组织裂解物中检测到PI3激酶调节亚基α亚型。
蛋白质印迹分析：0.5 µg/ml该抗体在10µg大鼠脑组织裂解物中检测到PI3激酶调节亚基α亚型。
Application
使用该兔多克隆抗PI3激酶p85，N-SH2结构域，目录号ABS233检测PI3激酶调节亚基α，已验证可用于免疫沉淀和蛋白质印迹。
免疫沉淀分析：5 µg代表性批次免疫沉淀来自200 µg大鼠脑组织裂解物的PI3激酶调节亚基α亚型。
免疫沉淀分析：5 µg代表性批次免疫沉淀来自500 µg Jurkat细胞裂解物的PI3激酶调节亚基α亚型。
蛋白质印迹分析：2 µg代表性批次在10 µg Jurkat细胞裂解物中检测到PI3激酶调节亚基α亚型。
免疫沉淀分析：5 µg代表性批次免疫沉淀来自500 µg Jurkat细胞裂解物的PI3激酶调节亚基α亚型。
蛋白质印迹分析：2 µg代表性批次在10 µg Jurkat细胞裂解物中检测到PI3激酶调节亚基α亚型。
研究子类别
发育信号转导
发育信号转导
研究类别
信号传导
信号传导
Biochem/physiol Actions
该多克隆抗体靶向UniProt（P27986，P26450，Q63787）报告的人（亚型1，AS53，p46，p85I），小鼠和大鼠（亚型p85-α，p55-α和p50-α）PI3激酶调节亚基α的剪接亚型共有的第一个SH2结构域区域。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
磷脂酰肌醇3-激酶调节亚基α（UniProt Q63787；也称为磷脂酰肌醇3-激酶85kDa调节亚基α，PI3-激酶调节亚基α，PtdIns-3-激酶调节亚基p85-α，PI3-激酶亚基p85-α，PI3K调节亚基α，PtdIns-3-激酶调节亚基α）由大鼠物种中的Pik3r1基因（基因ID 25513）编码。PI3K调节亚基α催化PI（4，5）P2肌醇环在3位的磷酸化，生成PI（3，4，5）P3，这是一种有效的第二信使，可介导生存信号和胰岛素作用。PI3激酶是由85 kDa调节亚基和110 kDa催化亚基组成的异二聚体复合物。配体结合后，生长因子受体的酪氨酸磷酸化产生p85结合的停泊位点（通过其SH2结构域），并充当衔接子，介导p110催化单元与质膜的关联性。在胰岛素刺激的胰岛素敏感性组织的葡萄糖摄取和糖原合成增加中起作用。已知Pik3r1基因的突变与抗胰岛素性有关。
观察值〜85/50 kDa。83.47/53.49/49.97/84.47 kDa（人亚型1/AS53/p46/p85I；UniProt P27986），83.39 kDa（小鼠；UniProt P26450），83.40/53.39/50.00 kDa（大鼠p85-α/p55-α/p50-α亚型；UniProt Q63787）。在某些裂解物中可以观察到未鉴定的条带。
Immunogen
GST标记的重组片段，对应于大鼠PI3激酶调节亚基α的第一个SH2结构域。
表位：第一个SH2结构域。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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